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PDCD4 抗体 (pSer457)

This 兔 多克隆 antibody specifically detects PDCD4 in WB, ELISA, IHC 和 IHC (p). It exhibits reactivity toward 人, 小鼠, 大鼠 和 非洲爪蟾.
产品编号 ABIN214444
发货至: 中国
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Quick Overview for PDCD4 抗体 (pSer457) (ABIN214444)

抗原

See all PDCD4 抗体
PDCD4 (Programmed Cell Death 4 (PDCD4))

适用

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人, 小鼠, 大鼠, 非洲爪蟾

宿主

  • 106
  • 8
  • 2

克隆类型

  • 90
  • 26
多克隆

标记

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This PDCD4 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

    • 15
    • 9
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    • 1
    pSer457

    特异性

    Amino acids 447-465 of human Pdcd4 protein.

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide from human PDCD4.

    Type of Immunogen: Synthetic peptide
  • 应用备注

    Approved: ELISA (1:1500 - 1:7000), IHC, IHC-P (1.25 - 2.5 μg/mL), WB (1:200 - 1:5000)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1.25-2.5 μg/mL.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    PDCD4 (Programmed Cell Death 4 (PDCD4))

    别名

    PDCD4

    背景

    Name/Gene ID: PDCD4
    Family: Apoptosis

    Synonyms: PDCD4, H731, Nuclear antigen H731-like, Nuclear antigen H731, Protein 197/15a

    基因ID

    27250

    UniProt

    Q53EL6
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