SAE1 抗体
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北京 101111
Quick Overview for SAE1 抗体 (ABIN214379)
抗原
See all SAE1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding to full length Human SUMO Activating Enzyme E1 fused with GST.
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纯化方法
- Protein A purified
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免疫原
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Recombinant human SAE1.
Type of Immunogen: Recombinant protein
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应用备注
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Approved: ELISA (1:5000 - 1:20000), IF, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- After adding water, will consist of 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide
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浓度
- Lot specific
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缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
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别名
- SAE1
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背景
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Name/Gene ID: SAE1
Synonyms: SAE1, Activator of SUMO1, AOS1, UBLE1A, HSPC140, SUA1 -
基因ID
- 10055
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UniProt
- Q9UBE0
抗原
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