Fibronectin 抗体
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北京 101111
Quick Overview for Fibronectin 抗体 (ABIN2141941)
抗原
See all Fibronectin 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody is specific for Human Factor XIII.
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交叉反应 (详细)
- This antibody reacts with Human Fibronectin. Ths antibody has negligible cross reactivity with Type I,II,III,IV,V,VI Collagens or Laminin1.0 mg/ml
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纯化方法
- Affinity chromatography purified
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过滤
- Sterile filtered
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免疫原
- Fibronectin antibody was raised in rabbit using fibronectin purified from human plasma as the immunogen.
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应用备注
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ELISA: 1:5,000-1:10,000, IHC: 1:50-1:200, IP: 1:100, WB: 1:5,000-1:10,000
Optimal conditions should be determined by the investigator. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Sterile filtered liquid In 0.02 M K3PO4, pH 7.2, with 0.15 M NaCl, and 0.01 % Sodium azide.
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注意事项
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. -
储存条件
- 4 °C/-20 °C
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储存方法
- Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
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- Fibronectin
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背景
- Human fibronectin has a molecular weight of 450,000 daltons when purified in an intact form from plasma. Fibronectin is a glycoprotein synthesized in the liver for the circulating blood plasma form, and is synthesized by many mesenchymal cells, for the extracellular matrix form. It is composed of two similar, but not identical protein chains, which are linked by two disulfide linkages at the C-terminal end of the chains. The chains are composed of domains which have specific secondary structures linked together by regions which are especially susceptible to proteolytic action. For this reason, detection by immunoblot (western) may show considerable variability in the observed apparent molecular weights, which is predicated on the source of the fibronectin, and to what degree proteolysis has occurred. Bands approximately 225 kDa should be observed after SDS-PAGE when reducing and denaturing conditions are used.
抗原
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