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NEK2 抗体 (AA 287-299)

The 兔 多克隆 anti-NEK2 antibody (ABIN213864) specifically detects NEK2 in WB, ELISA, IHC, IF 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN213864
发货至: 中国
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Quick Overview for NEK2 抗体 (AA 287-299) (ABIN213864)

抗原

See all NEK2 抗体
NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

适用

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宿主

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克隆类型

  • 80
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多克隆

标记

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This NEK2 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    AA 287-299

    特异性

    Aa 287-299 of Human NEK2 protein.

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide from human NEK2.

    Type of Immunogen: Synthetic peptide
  • 应用备注

    Approved: ELISA (1:2000 - 1:10000), IF, IHC, IHC-P (2.5 μg/mL), WB (1:500 - 1:2000)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid cycles of freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
  • 抗原

    NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

    别名

    NEK2

    背景

    Name/Gene ID: NEK2
    Subfamily: NEK
    Family: Protein Kinase

    Synonyms: NEK2, NIMA-related kinase 2, NEK2A, HsPK21, NimA-like protein kinase 1, NLK1, HSPK 21, NimA-related protein kinase 2

    基因ID

    4751

    UniProt

    P51955

    途径

    M Phase
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