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Vimentin 抗体

The 小鼠 单克隆 anti-Vimentin antibody is suitable to detect Vimentin in samples from 人, 大鼠, Pig, Cow, 猴, 小鸡, 犬 和 Cat. It has been validated for WB, IHC, IHC (p) 和 ICC.
产品编号 ABIN213302
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Vimentin 抗体 (ABIN213302)

抗原

See all Vimentin (VIM) 抗体
Vimentin (VIM)

适用

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人, 大鼠, Pig, Cow, 猴, 小鸡, 犬, Cat

宿主

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小鼠

克隆类型

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单克隆

标记

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This Vimentin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
  • 特异性

    Recognizes a 57-60kD protein, human Vimentin. Shows no cross-reactivity with other closely related intermediate filament proteins including desmin and GFAP. Species cross-reactivity: monkey, bovine, canine, feline, chicken and rat. Does not recognize mouse.

    纯化方法

    Purified

    免疫原

    Purified vimentin from porcine eye lens.

    Type of Immunogen: Purified protein

    亚型

    IgG1
  • 应用备注

    Approved: ICC (5 - 10 μg/mL), IHC, IHC-P, WB (1:1000)

    Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: 5-10 μg/mL, Can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antibody activity. Immunocytochemistry: 5-10 μg/mL. Positive control: Breast carcinoma tissue, colon, prostate, tonsil, tonsilar lymphoma, uterus and ureter.

    说明

    Target Species of Antibody: Pig

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.2, 0.1 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    4°C or -20°C, Avoid freeze-thaw cycles.
  • 抗原

    Vimentin (VIM)

    别名

    Vimentin

    背景

    Name/Gene ID: VIM

    Synonyms: VIM, Vimentin

    基因ID

    7431

    UniProt

    P08670

    途径

    Caspase Cascade in Apoptosis
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