LCK 抗体 (AA 1-58)
Quick Overview for LCK 抗体 (AA 1-58) (ABIN211979)
抗原
See all LCK 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-58
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特异性
- Recognizes Lck, Mr 56kD of mouse, human and bovine, reactivity with other Src family members not determined.
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纯化方法
- Protein A purified
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免疫原
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GST fusion protein corresponding to amino acids 1-58 of human Lck.
Type of Immunogen: Fusion protein -
亚型
- IgG
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应用备注
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Approved: IP, WB
Usage: Western Blot Analysis: 0.5-2 μg/mL detected Lck in RIPA lysates from Jurkat cells. Included Positive Antigen Control: Jurkat cell lysate. Use 20 μg per lane for minigels. Immunoprecipitation: 4 μg immunoprecipitated Lck from 1 μg of Jurkat RIPA lysate as confirmed by subsequent immunoblot using monoclonal anti-Lck. Western Blot Analysis Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Lck (1 μg/mL). Proteins were visualized using a goat anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
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别名
- p56lck / LCK
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背景
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Name/Gene ID: LCK
Subfamily: Src
Family: Protein Kinase
Synonyms: LCK, Lck kinase, LSK, p56-LCK, Pp58lck, Tyrosine-protein kinase Lck, p56lck, Protein YT16, Proto-oncogene Lck, YT16 -
基因ID
- 3932
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UniProt
- P06239
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
抗原
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