CD58 抗体 (Cytoplasmic Domain)
Quick Overview for CD58 抗体 (Cytoplasmic Domain) (ABIN211812)
抗原
See all CD58 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Cytoplasmic Domain
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特异性
- CD58, or LFA-3, is a membrane glycoprotein of 55-70kD. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes[1]. It is a member of the immunoglobulin superfamily of molecules. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells[2]. There are approximately 5000 CD58 Molecules on each erythrocyte. There is reduced expression of CD58 on hemopoietic cells in individuals with paroxysmal nocturnal hemoglobinuria. BRIC5 was produced in response to erythrocytes. The functional affinity of BRIC5 binding to erythrocytes is 4 x 108 M-1. Reacts by immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect hemagglutinin. The antigen on erythrocytes is Pronase sensitive. BRIC5 inhibits T cell rosetting.
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纯化方法
- Protein G purified
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免疫原
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Human erythrocytes.
Type of Immunogen: Cells -
亚型
- IgG2a
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anti-CD58 (CD58) (AA 1-240) antibody
CD58 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 2D11-B10 unconjugated
anti-CD58 (CD58) (AA 1-214) antibodyCD58 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 31-130) antibodyCD58 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 40-220) antibodyCD58 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 1-241) antibodyCD58 适用: 人 WB, ELISA, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 2D11-B10 unconjugated
anti-CD58 (CD58) antibodyCD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 unconjugated
anti-CD58 (CD58) antibody (Biotin)CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 Biotin
anti-CD58 (CD58) antibody (APC)CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 APC
anti-CD58 (CD58) antibody (PE)CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 PE
anti-CD58 (CD58) (AA 29-215) antibodyCD58 适用: 人 FACS, WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: Flo (1:10), WB
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.
Not recommended for: IHC-Fr, IHC-P -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- 0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- CD58
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别名
- CD58
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背景
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Name/Gene ID: CD58
Family: Immunoglobulin
Synonyms: CD58, CD58 antigen, AG3, LFA-3, LFA3, Surface glycoprotein LFA-3, Surface glycoprotein lfa3, CD58 Molecule -
基因ID
- 965
抗原
-
