CBL 抗体 (AA 878-906)
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- 抗原 See all CBL 抗体
- CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))
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抗原表位
- AA 878-906
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适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CBL antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 特异性
- Specific for Cbl. Species cross-reactivity: mouse and rat.
- 纯化方法
- Protein G purified
- 免疫原
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GST fusion protein corresponding to residues 878-906 of human Cbl. This sequence is identical in mouse.
Type of Immunogen: Fusion protein - 亚型
- IgG
- Top Product
- Discover our top product CBL Primary Antibody
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- 应用备注
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Approved: ICC (5 - 10 μg/mL), IP, WB
Usage: Suitable for use in Western Blot, Immunoprecipitation, and Immunocytochemistry. Western Blot Analysis: 0.5-2 μg/mL detects Cbl (120kD) in RIPA lysates from human Jurkat and mouse 3T3 cells. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Cbl (1 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 2 μg of a previous lot immunoprecipitated Cbl from 1 μg of a mouse 3T3 cell RIPA lysate. Immunocytochemistry: 5-10 μg/mL of a previous lot showed positive immunostaining for Cbl in 3T3 cells fixed with ice cold methanol/ethanol [1:1] for 1 minute. Positive control: F4209:3T3 lysate. Use 20 μg per lane for minigels. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, 20 % glycerol, 0.05 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))
- 别名
- c-CBL (CBL 产品)
- 别名
- 4732447J05Rik antibody, Cbl-2 antibody, c-Cbl antibody, cbl antibody, C-CBL antibody, CBL2 antibody, FRA11B antibody, NSLL antibody, RNF55 antibody, c-cbl-like antibody, RGD1561386 antibody, Casitas B-lineage lymphoma antibody, Cbl proto-oncogene antibody, Cbl antibody, CBL antibody
- 背景
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Name/Gene ID: CBL
Synonyms: CBL, C-CBL, Oncogene CBL2, RING finger protein 55, Proto-oncogene c-Cbl, RNF55, CBL2, FRA11B, NSLL - 基因ID
- 867
- UniProt
- P22681
- 途径
- TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals
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