CBL 抗体 (AA 878-906)
Quick Overview for CBL 抗体 (AA 878-906) (ABIN211561)
抗原
See all CBL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 878-906
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特异性
- Specific for Cbl. Species cross-reactivity: mouse and rat.
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纯化方法
- Protein G purified
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免疫原
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GST fusion protein corresponding to residues 878-906 of human Cbl. This sequence is identical in mouse.
Type of Immunogen: Fusion protein -
亚型
- IgG
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应用备注
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Approved: ICC (5 - 10 μg/mL), IP, WB
Usage: Suitable for use in Western Blot, Immunoprecipitation, and Immunocytochemistry. Western Blot Analysis: 0.5-2 μg/mL detects Cbl (120kD) in RIPA lysates from human Jurkat and mouse 3T3 cells. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Cbl (1 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 2 μg of a previous lot immunoprecipitated Cbl from 1 μg of a mouse 3T3 cell RIPA lysate. Immunocytochemistry: 5-10 μg/mL of a previous lot showed positive immunostaining for Cbl in 3T3 cells fixed with ice cold methanol/ethanol [1:1] for 1 minute. Positive control: F4209:3T3 lysate. Use 20 μg per lane for minigels. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, 20 % glycerol, 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))
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别名
- c-CBL
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背景
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Name/Gene ID: CBL
Synonyms: CBL, C-CBL, Oncogene CBL2, RING finger protein 55, Proto-oncogene c-Cbl, RNF55, CBL2, FRA11B, NSLL -
基因ID
- 867
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UniProt
- P22681
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途径
- TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals
抗原
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