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Vinculin 抗体

This anti-Vinculin antibody is a 小鼠 单克隆 antibody detecting Vinculin in WB, IHC, IP, ICC 和 IHC (fro). Suitable for 人, 小鼠, 大鼠, 兔 和 小鸡.
产品编号 ABIN211429
发货至: 中国

Quick Overview for Vinculin 抗体 (ABIN211429)

抗原

See all Vinculin (VCL) 抗体
Vinculin (VCL)

适用

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人, 小鼠, 大鼠, 兔, 小鸡

宿主

  • 100
  • 52
  • 1
小鼠

克隆类型

  • 87
  • 66
单克隆

标记

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This Vinculin antibody is un-conjugated

应用范围

  • 115
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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 特异性

    Recognizes human vinculin at 117kD. Species cross-reactivity: rat, mouse, rabbit and chicken.

    纯化方法

    Protein A purified

    免疫原

    Human vinculin.

    Type of Immunogen: Purified protein

    亚型

    IgG1
  • 应用备注

    Approved: ICC, IHC, IHC-Fr, IP, WB (0.1 - 1 μg/mL)

    Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitates Vinculin from mouse 3T3/A31 and human A431 RIPA lysates. Immunohistochemistry and Immunocytochemistry (frozen and formalin fixed sections): 10 μg/mL will stain Vinculin in Ref-52 cells fixed with 4 % paraformaldehyde and permeabilized with 0.25 % Triton X-100.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.05 % sodium azide, 1 mg/mL BSA, 40 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    Vinculin (VCL)

    别名

    VCL / Vinculin

    背景

    Name/Gene ID: VCL
    Family: Cytoskeleton

    Synonyms: VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL

    基因ID

    7414

    UniProt

    P18206

    途径

    Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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