Vinculin 抗体
Quick Overview for Vinculin 抗体 (ABIN211429)
抗原
See all Vinculin (VCL) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes human vinculin at 117kD. Species cross-reactivity: rat, mouse, rabbit and chicken.
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纯化方法
- Protein A purified
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免疫原
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Human vinculin.
Type of Immunogen: Purified protein -
亚型
- IgG1
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应用备注
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Approved: ICC, IHC, IHC-Fr, IP, WB (0.1 - 1 μg/mL)
Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitates Vinculin from mouse 3T3/A31 and human A431 RIPA lysates. Immunohistochemistry and Immunocytochemistry (frozen and formalin fixed sections): 10 μg/mL will stain Vinculin in Ref-52 cells fixed with 4 % paraformaldehyde and permeabilized with 0.25 % Triton X-100. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.05 % sodium azide, 1 mg/mL BSA, 40 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Vinculin (VCL)
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别名
- VCL / Vinculin
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背景
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Name/Gene ID: VCL
Family: Cytoskeleton
Synonyms: VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL -
基因ID
- 7414
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UniProt
- P18206
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途径
- Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
抗原
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