FYB 抗体 (AA 755-768)
Quick Overview for FYB 抗体 (AA 755-768) (ABIN211342)
抗原
See all FYB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 755-768
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特异性
- Recognizes ADAP/SLAP-130/Fyb, Mr ~130kD. An unknown protein was also detected, Mr ~40kD that is also inhibited by the immunizing peptide. Species sequence homology: Human and rat.
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预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Horse, Pig (100%) Platypus (93%).
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纯化方法
- Protein A purified
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免疫原
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Synthetic peptide corresponding to amino acids 755-768 (DNDGEIYDDIADGC) of mouse Fyn binding protein (ADAP/SLAP-130/Fyb) (KLH). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Horse, Pig (100%), Platypus (93%).
Type of Immunogen: Synthetic peptide - KLH conjugated -
亚型
- IgG
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应用备注
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Approved: IP, WB (0.5 - 2 μg/mL)
Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitated ADAP/SLAP-130/Fyb from 500 μg of RAW. 264.7 macrophage RIPA lysate.
Not recommended for: IHC-P -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, before the addition of glycerol to 30 %
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- FYB (FYN-Binding Protein (FYB))
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别名
- ADAP / FYB
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背景
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Name/Gene ID: FYB
Synonyms: FYB, ADAP, FYN-binding protein, FYN-T-binding protein, FYB-120/130, FYN binding protein, p120/p130, PRO0823, SLAP130, SLAP-130 -
基因ID
- 2533
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UniProt
- O15117
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途径
- TCR Signaling, Protein targeting to Nucleus
抗原
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