Calreticulin 抗体 (C-Term)
Quick Overview for Calreticulin 抗体 (C-Term) (ABIN211075)
抗原
See all Calreticulin (CALR) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 412-417, C-Term
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特异性
- Specific for calreticulin. Does not cross-react with other endoplasmic reticulum proteins. Species cross-reactivity: Human, mouse, rat, Xenopus and weakly with bovine and porcine. No reaction with chicken.
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纯化方法
- Protein A purified
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免疫原
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Synthetic peptide (QAKDEL) corresponding to the C-terminus (amino acids 412-417) of human calreticulin, KLH conjugated.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: IHC, WB (0.5 - 2 μg/mL)
Usage: Suitable for Western Blot and Immunohistochemistry. Western Blot: 0.5-2 μg/mL detects calreticulin in RIPA lysates from murine 3T3/NIH fibroblasts and human foreskin fibroblast (HFF). 20 μg 3T3/NIH and 10 μg HFF cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-human Calreticulin (0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: Detects calreticulin in tissue sections and cells. Included Positive Antigen Control: 3T3 lysate. Use 20 μg per lane for minigels. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Frozen solution in 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Calreticulin (CALR)
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别名
- CALR / Calreticulin
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背景
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Name/Gene ID: CALR
Synonyms: CALR, Calregulin, CC1qR, CRP55, CRT, CRTC, ERp60, Grp60, RO, SSA, Calreticulin, HACBP -
基因ID
- 811
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UniProt
- P27797
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途径
- Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response
抗原
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