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Calreticulin 抗体 (C-Term)

This 兔 多克隆 antibody specifically detects Calreticulin in WB 和 IHC. It exhibits reactivity toward 人, 小鼠, 大鼠, Pig, Cow 和 非洲爪蟾.
产品编号 ABIN211075
发货至: 中国
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北京 101111
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Room 801-803
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Quick Overview for Calreticulin 抗体 (C-Term) (ABIN211075)

抗原

See all Calreticulin (CALR) 抗体
Calreticulin (CALR)

适用

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人, 小鼠, 大鼠, Pig, Cow, 非洲爪蟾

宿主

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克隆类型

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多克隆

标记

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This Calreticulin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)
  • 抗原表位

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    AA 412-417, C-Term

    特异性

    Specific for calreticulin. Does not cross-react with other endoplasmic reticulum proteins. Species cross-reactivity: Human, mouse, rat, Xenopus and weakly with bovine and porcine. No reaction with chicken.

    纯化方法

    Protein A purified

    免疫原

    Synthetic peptide (QAKDEL) corresponding to the C-terminus (amino acids 412-417) of human calreticulin, KLH conjugated.

    Type of Immunogen: Synthetic peptide

    亚型

    IgG
  • 应用备注

    Approved: IHC, WB (0.5 - 2 μg/mL)

    Usage: Suitable for Western Blot and Immunohistochemistry. Western Blot: 0.5-2 μg/mL detects calreticulin in RIPA lysates from murine 3T3/NIH fibroblasts and human foreskin fibroblast (HFF). 20 μg 3T3/NIH and 10 μg HFF cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-human Calreticulin (0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: Detects calreticulin in tissue sections and cells. Included Positive Antigen Control: 3T3 lysate. Use 20 μg per lane for minigels.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    Frozen solution in 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    Calreticulin (CALR)

    别名

    CALR / Calreticulin

    背景

    Name/Gene ID: CALR

    Synonyms: CALR, Calregulin, CC1qR, CRP55, CRT, CRTC, ERp60, Grp60, RO, SSA, Calreticulin, HACBP

    基因ID

    811

    UniProt

    P27797

    途径

    Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response
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