VCAM1 抗体
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- 抗原 See all VCAM1 抗体
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This VCAM1 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Reacts with human VCAM-1.
- 纯化方法
- Purified
- 免疫原
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Type of Immunogen: Native protein - 亚型
- IgG1
- Top Product
- Discover our top product VCAM1 Primary Antibody
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anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 651-739) antibody
VCAM1 适用: 人, 小鼠, 大鼠, 兔, 绵羊 WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 1-739) antibodyVCAM1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 1-739) antibodyVCAM1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) antibodyVCAM1 适用: 人, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3H10 unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-183) antibodyVCAM1 适用: 人 FACS, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 6A11D8 unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 109-318) antibodyVCAM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 300-400) antibodyVCAM1 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) antibodyVCAM1 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 6G9 unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 511-682) antibodyVCAM1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 409-682) antibodyVCAM1 适用: 兔 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
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- 应用备注
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Approved: Flo (1:10), IHC, IHC-Fr, IP
Usage: Useful for staining of VCAM-1 expressing endothelial cells. It permits staining of in vitro cultured cells and frozen tissue sections. Furthermore the antibody can be used for Flow Cytometry, immunoassays and Immunoprecipitation. Flow Cytometry: 1:10. Immunohistology: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. In vitro cultured cells can be fixed with 1 % paraformaldehyde, tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/mL), IL-1 (100U/mL) or LPS (1 μg/mL) in tissue culture medium at 37?C. As negative control it is advised to use a control murine IgG1 antibody. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, 0.02 % sodium azide, 0.1 % BSA.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- 抗原
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
- 别名
- VCAM1 / VCAM-1 / CD106 (VCAM1 产品)
- 背景
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Name/Gene ID: VCAM1
Family: ICAM
Synonyms: VCAM1, CD106, CD106 antigen, INCAM-100, V-CAM 1, VCAM-1 - 基因ID
- 7412
- UniProt
- P19320
- 途径
- Carbohydrate Homeostasis
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