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ZAP70 抗体 (C-Term)

This 小鼠 单克隆 antibody specifically detects ZAP70 in WB, FACS, ICC 和 Inhibition. It exhibits reactivity toward 人.
产品编号 ABIN207127
发货至: 中国
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Room 801-803
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Quick Overview for ZAP70 抗体 (C-Term) (ABIN207127)

抗原

See all ZAP70 抗体
ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

适用

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宿主

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小鼠

克隆类型

  • 190
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单克隆

标记

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This ZAP70 antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Blocking Antibody (Inhibition)

克隆位点

ZAP-03
  • 抗原表位

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    C-Term

    特异性

    Reacts with ZAP-70, a 70 kD protein tyrosine kinase expressed in T and NK cells. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.

    纯化方法

    Protein A purified

    免疫原

    Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70.

    Type of Immunogen: Fusion protein

    亚型

    IgG1
  • 应用备注

    Approved: Flo (2 - 5 μg/mL), ICC, Inhb, WB (5 μg/mL)

    Usage: Flow Cytometry: Recommended dilution - 2-5 μg/mL. Positive control -. HPB-ALL human peripheral blood T cell leukemia cell line. Application note - Intracellular staining. Western Blotting: Recommended dilution - 0,5 μg/mL. Positive control -. HPB-ALL huma

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, 15 mM sodium azide, approx,  pH 7.4.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    avoid freeze thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • 抗原

    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

    别名

    ZAP70

    背景

    Name/Gene ID: ZAP70
    Subfamily: Syk/ZAP70
    Family: Protein Kinase

    Synonyms: ZAP70, 70 kDa zeta-associated protein, SRK, STCD, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70, TZK, ZAP-70, Zeta-associated protein 70

    基因ID

    7535

    UniProt

    P43403

    途径

    TCR Signaling, Ubiquitin Proteasome Pathway
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