RFXANK 抗体
Quick Overview for RFXANK 抗体 (ABIN204403)
抗原
See all RFXANK 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Specific for s all splice forms of Ankyrin G containing a spectrin-binding domain. Does not cross-react with related ankyrin isoforms. Species cross-reactivity: Human, mouse, rabbit, rat, canine.
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纯化方法
- Protein A purified
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免疫原
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Synthetic peptide derived from the spectrin-binding domain of the human ankyrin G protein.
Type of Immunogen: Synthetic peptide -
亚型
- IgG1 kappa
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应用备注
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Approved: ELISA (0.1 - 1 μg/mL), IF, IHC, IHC-Fr, IP, WB (1 - 3 μg/mL)
Usage: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. ELISA (native): 0.1-1.0 μg/mL. Detects a band between ~200-300kD. Western Blot: 1-3 μg/mL. For best results on rat brain, mouse brain or MDCK cell lysates, add 0.3 M sucrose to incubation buffer. Immunofluorescence (frozen sections): 5-10 μg/mL. Sensitive to fixation conditions. Recommend 1 % paraformaldehyde for perfusion and 4 hrs of post fixation, after perfusion. For sciatic nerve, recommend 2 % paraformaldehyde for 2 hrs at 4?C. Immunoprecipitation (native): 5 μg/ IP. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.2, 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- RFXANK (Regulatory Factor X-Associated Ankyrin Containing Protein (RFXANK))
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别名
- RFXANK
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背景
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Name/Gene ID: RFXANK
Synonyms: RFXANK, ANKRA1, BLS, DNA-binding protein RFXANK, F14150_1, RFXB, RFX-B, Regulatory factor X subunit B, RFX-Bdelta4 -
基因ID
- 8625
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UniProt
- O14593
抗原
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