ITGB1 抗体
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- 抗原 See all ITGB1 抗体
- ITGB1 (Integrin beta 1 (ITGB1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ITGB1 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
- 特异性
- Recognizes human Integrin beta 1. Reacts with calcium-dependent epitopes on the integrin b1 subunit. When bound to b1, activates b1 dependent binding to extra cellular matrix substrates. Species cross-reactivity: Rabbit and monkey.
- 纯化方法
- Protein A purified
- 免疫原
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Human IL-1b activated endothelial cells.
Type of Immunogen: Cells - 亚型
- IgG1
- Top Product
- Discover our top product ITGB1 Primary Antibody
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- 应用备注
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Approved: ELISA, Flo, ICC (2 μg/mL), IHC, IHC-Fr, IHC-P, IP
Usage: Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, FACS and ELISA. Immunocytochemistry: 2 μg/mL (use on numerous cell lines as long as Ca2+ is present),. Immunoprecipitation: 5 μg/400 μL lysate (0.5 % triton PBS plus Ca++). FACS: 2-5 μg/1 x106, in the presence of Ca++. Stimulation of cells: 2-10 μg/mL per 1 x106. Binding buffer must contain 1 mM Ca++. Immunohistochemistry: For use on acetone or mild paraformaldehyde fixed tissue (2-2.5 % PFA, short time) frozen and paraffin embedded (1:500, citrate HEIR treated, In et al 2003). Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.1. No preservatives.
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short-term: 4°C, Long-term: Add sterile 40-50% glycerol, aliquot, and store at -20°C.
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- 抗原
- ITGB1 (Integrin beta 1 (ITGB1))
- 别名
- ITGB1 / Integrin Beta 1 / CD29 (ITGB1 产品)
- 背景
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Name/Gene ID: ITGB1
Family: Integrin
Synonyms: ITGB1, CD29, CD29 antigen, Integrin beta-1, MDF2, MSK12, Integrin VLA-4 beta subunit, VLA-BETA, VLAB, Beta 1 integrin, FNRB, GPIIA, Integrin Beta 1, VLA-4 subunit beta - 基因ID
- 3688
- UniProt
- P05556
- 途径
- Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome
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