ITGB1 抗体
Quick Overview for ITGB1 抗体 (ABIN204312)
抗原
See all ITGB1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes human Integrin beta 1. Reacts with calcium-dependent epitopes on the integrin b1 subunit. When bound to b1, activates b1 dependent binding to extra cellular matrix substrates. Species cross-reactivity: Rabbit and monkey.
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纯化方法
- Protein A purified
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免疫原
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Human IL-1b activated endothelial cells.
Type of Immunogen: Cells -
亚型
- IgG1
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应用备注
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Approved: ELISA, Flo, ICC (2 μg/mL), IHC, IHC-Fr, IHC-P, IP
Usage: Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, FACS and ELISA. Immunocytochemistry: 2 μg/mL (use on numerous cell lines as long as Ca2+ is present),. Immunoprecipitation: 5 μg/400 μL lysate (0.5 % triton PBS plus Ca++). FACS: 2-5 μg/1 x106, in the presence of Ca++. Stimulation of cells: 2-10 μg/mL per 1 x106. Binding buffer must contain 1 mM Ca++. Immunohistochemistry: For use on acetone or mild paraformaldehyde fixed tissue (2-2.5 % PFA, short time) frozen and paraffin embedded (1:500, citrate HEIR treated, In et al 2003). Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.1. No preservatives.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Short-term: 4°C, Long-term: Add sterile 40-50% glycerol, aliquot, and store at -20°C.
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- ITGB1 (Integrin beta 1 (ITGB1))
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别名
- ITGB1 / Integrin Beta 1 / CD29
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背景
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Name/Gene ID: ITGB1
Family: Integrin
Synonyms: ITGB1, CD29, CD29 antigen, Integrin beta-1, MDF2, MSK12, Integrin VLA-4 beta subunit, VLA-BETA, VLAB, Beta 1 integrin, FNRB, GPIIA, Integrin Beta 1, VLA-4 subunit beta -
基因ID
- 3688
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UniProt
- P05556
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途径
- Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome
抗原
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