ESAM 抗体
Quick Overview for ESAM 抗体 (ABIN204196)
抗原
See all ESAM 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Selected for its ability to detect human ESAM in direct ELISAs and Western Blots. In these applications, this antibody shows no cross-reactivity with rmESAM.
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纯化方法
- Protein G purified
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免疫原
- NS0-derived rhESAM.
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亚型
- IgG2b
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anti-Endothelial Cell Adhesion Molecule (ESAM) (AA 29-248) antibody
ESAM 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Endothelial Cell Adhesion Molecule (ESAM) (AA 21-120) antibodyESAM 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Endothelial Cell Adhesion Molecule (ESAM) (AA 1-390) antibodyESAM 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Endothelial Cell Adhesion Molecule (ESAM) (AA 30-248) antibodyESAM 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Endothelial Cell Adhesion Molecule (ESAM) (AA 30-248) antibodyESAM 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Endothelial Cell Adhesion Molecule (ESAM) (AA 1-390) antibodyESAM 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1G8 unconjugated
anti-Endothelial Cell Adhesion Molecule (ESAM) (AA 1-390) antibodyESAM 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Endothelial Cell Adhesion Molecule (ESAM) (AA 1-390) antibodyESAM 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1E4 unconjugated
anti-Endothelial Cell Adhesion Molecule (ESAM) (AA 30-248) antibodyESAM 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Endothelial Cell Adhesion Molecule (ESAM) (AA 31-390) antibodyESAM 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, Flo, ICC, WB
Usage: Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human ESAM. Using a colorimetric detection system, the detection limit for rhESAM is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using HUVECs. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. HUVECs were stained with anti-ESAM or isotype control antibody, followed by PE-conjugated anti-mouse antibody. Immunocytochemistry: This antibody was used at a concentration of 10 μg/mL to detect ESAM in HUVECs. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by NL557-coupled anti-mouse IgG in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human ESAM. The detection limit for rhESAM is approximately 1 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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- ESAM (Endothelial Cell Adhesion Molecule (ESAM))
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别名
- ESAM
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背景
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Name/Gene ID: ESAM
Synonyms: ESAM, LP4791 protein, W117m -
基因ID
- 90952
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UniProt
- Q96AP7
抗原
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