Chitotriosidase 1 抗体
Quick Overview for Chitotriosidase 1 抗体 (ABIN204118)
抗原
See all Chitotriosidase 1 (CHIT1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes human CHIT1 in direct ELISAs and Western Blots. In these formats, this antibody shows no cross-reactivity with rhCHI-3L1. Species sequence homology: mouse: 79 % .
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纯化方法
- Protein G purified
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免疫原
- NS0-derived rhCHIT-1.
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亚型
- IgG1
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应用备注
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Approved: ELISA, IP (25 μg/mL), Neut
Usage: Suitable for use in ELISA, Immunoprecipitation and Neutralization. ELISA (direct): 0.5-1.0 μg/mL. Detection limit for rhCHIT1 is approximately 2 ng/well. Immunoprecipitation: 25 μg/mL to immunoprecipitate rhCHIT1 from conditioned cell culture medium. Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 min. The enzyme was then assayed with 4-MU-beta-D-N, N', N''-triacetylchitotriose. 50 % of the enzymatic activity was inhibited by the antibody at approximately 56 ng/mL (IC 50) under conditions in which the enzyme was present at 20 ng/mL and the substrate concentration was 0.01 mM. IC 50 was achieved at approximately 1:1 Molar ratio of the antibody to the enzyme. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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- Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))
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别名
- CHIT1 / Chitotriosidase
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背景
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Name/Gene ID: CHIT1
Synonyms: CHIT1, CHITD, CHI3, Chitinase-1, Chitinase 1 (chitotriosidase), Chitotriosidase, CHIT, Chitotriosidase-1 -
基因ID
- 1118
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UniProt
- Q13231
抗原
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