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Chitotriosidase 1 抗体

This anti-Chitotriosidase 1 antibody is a 小鼠 单克隆 antibody detecting Chitotriosidase 1 in ELISA, IP 和 Neut. Suitable for 人 和 小鼠.
产品编号 ABIN204118
发货至: 中国

Quick Overview for Chitotriosidase 1 抗体 (ABIN204118)

抗原

See all Chitotriosidase 1 (CHIT1) 抗体
Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))

适用

  • 36
  • 8
  • 3
人, 小鼠

宿主

  • 32
  • 10
  • 1
小鼠

克隆类型

  • 35
  • 8
单克隆

标记

  • 31
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Chitotriosidase 1 antibody is un-conjugated

应用范围

  • 30
  • 18
  • 13
  • 10
  • 10
  • 2
  • 1
  • 1
  • 1
  • 1
ELISA, Immunoprecipitation (IP), Neutralization (Neut)
  • 特异性

    Recognizes human CHIT1 in direct ELISAs and Western Blots. In these formats, this antibody shows no cross-reactivity with rhCHI-3L1. Species sequence homology: mouse: 79 % .

    纯化方法

    Protein G purified

    免疫原

    NS0-derived rhCHIT-1.

    亚型

    IgG1
  • 应用备注

    Approved: ELISA, IP (25 μg/mL), Neut

    Usage: Suitable for use in ELISA, Immunoprecipitation and Neutralization. ELISA (direct): 0.5-1.0 μg/mL. Detection limit for rhCHIT1 is approximately 2 ng/well. Immunoprecipitation: 25 μg/mL to immunoprecipitate rhCHIT1 from conditioned cell culture medium. Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 min. The enzyme was then assayed with 4-MU-beta-D-N, N', N''-triacetylchitotriose. 50 % of the enzymatic activity was inhibited by the antibody at approximately 56 ng/mL (IC 50) under conditions in which the enzyme was present at 20 ng/mL and the substrate concentration was 0.01  mM. IC 50 was achieved at approximately 1:1 Molar ratio of the antibody to the enzyme.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    deionized water

    浓度

    Lot specific

    缓冲液

    Lyophilized.
  • 抗原

    Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))

    别名

    CHIT1 / Chitotriosidase

    背景

    Name/Gene ID: CHIT1

    Synonyms: CHIT1, CHITD, CHI3, Chitinase-1, Chitinase 1 (chitotriosidase), Chitotriosidase, CHIT, Chitotriosidase-1

    基因ID

    1118

    UniProt

    Q13231
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