CD58 抗体 (Biotin)
Quick Overview for CD58 抗体 (Biotin) (ABIN204009)
抗原
See all CD58 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Reacts with both transmembrane and GPI anchored CD58. Recognizes the CD58 glycoprotein of about 60-70kD and will inhibit HLA-DR mediated T cell cytotoxicity.
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纯化方法
- Protein A purified
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免疫原
- Human cytotoxic T cells.
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亚型
- IgG1
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应用备注
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Approved: Ctox, Flo, IHC, IHC-Fr, IP
Usage: Antibody will inhibit HLA-DR mediated T cell cytotoxicity. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation. Immunohistochemistry: Frozen. Flow Cytometry: 5x10^5 cultured human Nalm-6 cells were washed and incubated 45 minutes on ice with 80 mL of anti-CD58/Biotin at 10 mg/mL. Cells were washed twice and incubated with 2? reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.29 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was 94 % blocked when pre incubated 10 minutes with 20 mL of 0.5 mg/mL anti-CD58 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.2, 5 % glycerol, 0.2 % BSA, 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- CD58
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别名
- CD58
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背景
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Name/Gene ID: CD58
Family: Immunoglobulin
Synonyms: CD58, CD58 antigen, AG3, LFA-3, LFA3, Surface glycoprotein LFA-3, Surface glycoprotein lfa3, CD58 Molecule -
基因ID
- 965
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UniProt
- P19256
抗原
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