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CD58 抗体 (Biotin)

This anti-CD58 antibody is a 小鼠 单克隆 antibody detecting CD58 in FACS, IHC, IP, IHC (fro) 和 CyTox. Suitable for 人.
产品编号 ABIN204009
发货至: 中国

Quick Overview for CD58 抗体 (Biotin) (ABIN204009)

抗原

See all CD58 抗体
CD58

适用

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宿主

  • 46
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小鼠

克隆类型

  • 45
  • 36
单克隆

标记

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This CD58 antibody is conjugated to Biotin

应用范围

  • 44
  • 35
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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)
  • 特异性

    Reacts with both transmembrane and GPI anchored CD58. Recognizes the CD58 glycoprotein of about 60-70kD and will inhibit HLA-DR mediated T cell cytotoxicity.

    纯化方法

    Protein A purified

    免疫原

    Human cytotoxic T cells.

    亚型

    IgG1
  • 应用备注

    Approved: Ctox, Flo, IHC, IHC-Fr, IP

    Usage: Antibody will inhibit HLA-DR mediated T cell cytotoxicity. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation. Immunohistochemistry: Frozen. Flow Cytometry: 5x10^5 cultured human Nalm-6 cells were washed and incubated 45 minutes on ice with 80 mL of anti-CD58/Biotin at 10 mg/mL. Cells were washed twice and incubated with 2? reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.29 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was 94 % blocked when pre incubated 10 minutes with 20 mL of 0.5 mg/mL anti-CD58 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.2, 5 % glycerol, 0.2 % BSA, 0.05 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    CD58

    别名

    CD58

    背景

    Name/Gene ID: CD58
    Family: Immunoglobulin

    Synonyms: CD58, CD58 antigen, AG3, LFA-3, LFA3, Surface glycoprotein LFA-3, Surface glycoprotein lfa3, CD58 Molecule

    基因ID

    965

    UniProt

    P19256
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