CD58 抗体 (Biotin)
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- 抗原 See all CD58 抗体
- CD58
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD58 antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)
- 特异性
- Reacts with both transmembrane and GPI anchored CD58. Recognizes the CD58 glycoprotein of about 60-70kD and will inhibit HLA-DR mediated T cell cytotoxicity.
- 纯化方法
- Protein A purified
- 免疫原
- Human cytotoxic T cells.
- 亚型
- IgG1
- Top Product
- Discover our top product CD58 Primary Antibody
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anti-CD58 (CD58) (AA 1-240) antibody
CD58 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 2D11-B10 unconjugated
anti-CD58 (CD58) (AA 1-214) antibodyCD58 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 31-130) antibodyCD58 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 40-220) antibodyCD58 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 1-241) antibodyCD58 适用: 人 WB, ELISA, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 2D11-B10 unconjugated
anti-CD58 (CD58) antibodyCD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 unconjugated
anti-CD58 (CD58) antibody (Biotin)CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 Biotin
anti-CD58 (CD58) antibody (PE)CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 PE
anti-CD58 (CD58) antibody (APC)CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 APC
anti-CD58 (CD58) (AA 29-215) antibodyCD58 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: Ctox, Flo, IHC, IHC-Fr, IP
Usage: Antibody will inhibit HLA-DR mediated T cell cytotoxicity. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation. Immunohistochemistry: Frozen. Flow Cytometry: 5x10^5 cultured human Nalm-6 cells were washed and incubated 45 minutes on ice with 80 mL of anti-CD58/Biotin at 10 mg/mL. Cells were washed twice and incubated with 2? reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.29 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was 94 % blocked when pre incubated 10 minutes with 20 mL of 0.5 mg/mL anti-CD58 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.2, 5 % glycerol, 0.2 % BSA, 0.05 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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