GDF2 抗体 (AA 320-429)
Quick Overview for GDF2 抗体 (AA 320-429) (ABIN203805)
抗原
See all GDF2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 320-429
-
特异性
- Recognizes human Bone Morphogenetic Protein 9. Does not cross react with recombinant human BMP-1, -1.1, -2, -3, -3b, -4, -5, -6, -7, -8 or -10. In direct ELISAs, ~80 % cross-reactivity with recombinant mouse BMP-9 and 10 % cross-reactivity with recombinant mouse BMP-10 is observed.
-
纯化方法
- Affinity purified
-
过滤
- 0.2 μm filtered
-
免疫原
-
Recombinant protein corresponding to aa320-429 from human BMP-9, expressed in E.coli (UniProt# Q9UK05).
Type of Immunogen: Recombinant protein -
亚型
- IgG2b
-
-
-
-
应用备注
-
Approved: ELISA (0.5 - 1 μg/mL), Func, Neut, WB (1 - 2 μg/mL)
Usage: Suitable for use in Western Blot, ELISA and Neutralization. Western Blot: 1-2 μg/mL, The detection limit is ~5 ng/lane under non-reducing conditions. Direct ELISA: 0.5-1.0 μg/mL, The detection limit is ~5 ng/well. Neutralization of human BMP-9 bioactivity: The ND50 is defined as the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when the cytokine is present at a concentration just high enough to elicit maximum response. The ND50 of this lot is ~0.01-0.04 μg/mL in the presence of 2 ng/mL rhBMP-9 using ATDC5 chondrogenic cells. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Sterile PBS
-
浓度
- Lot specific
-
缓冲液
- Lyophilized from a 0.2 um filtered solution in PBS, 5 % trehalose.
-
注意事项
- Avoid frequent freeze thaws. Store undiluted.
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Store at -20°C to -70°C. Stable for 1 year from date of receipt.
-
-
- GDF2 (Growth Differentiation Factor 2 (GDF2))
-
别名
- GDF2 / BMP9
-
背景
-
Name/Gene ID: GDF2
Family: TGF beta
Synonyms: GDF2, BMP-9, BMP9, GDF-2, Bone morphogenetic protein 9 -
基因ID
- 2658
-
UniProt
- Q9UK05
-
途径
- Transition Metal Ion Homeostasis
抗原
-