Plakophilin 3 抗体
Quick Overview for Plakophilin 3 抗体 (ABIN201418)
抗原
See all Plakophilin 3 (PKP3) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Reacts with the 87kD human Plakophilin-3. There is no cross reactivity with human Plakophilin-1 and Plakophilin-2 proteins. Reactivity is confirmed with human A431 cells, and HEK293 cells transfected with plasmid expressing human Plakophilin-3. Based on sequence homology, this antibody is expected to react with mouse and canine.
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纯化方法
- Protein A purified
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免疫原
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Synthetic peptide from Armadillo domain of human Plakophilin-3.
Type of Immunogen: Synthetic peptide -
亚型
- IgG2b
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应用备注
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Approved: ELISA (0.1 - 1 μg/mL), IF (1 - 3 μg/mL), IHC, IHC-P, IP, WB (1 - 3 μg/mL)
Usage: Suitable for use in ELISA, Immunoprecipitation (native), Immunohistochemistry (formalin fixed paraffin embedded), Immunofluorescence and Western Blot. ELISA: 0.1-1 μg/mL. Immunoprecipitation: 10 μg/IP reaction. Immunohistochemistry: 10 μg/mL. Immunofluorescence: 1-3 μg/mL. Western Blot: 1-3 μg/mL. Note: Immunofluorescent assays were performed using methanol-fixed HCT8/E8 and paraformaldehyde-fixed HaCat cells. For optimal IF results, methanol and paraformaldehyde- fixed cells should be permeabilized with 0.2 % Triton X-100 for 5 and 15 minutes, respectively, prior to incubation with primary antibody. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Plakophilin 3 (PKP3)
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别名
- PKP3 / Plakophilin 3
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背景
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Name/Gene ID: PKP3
Synonyms: PKP3, Plakophilin 3, Plakophilin-3, Plakophilin 3b -
基因ID
- 11187
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UniProt
- Q9Y446
抗原
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