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Plakophilin 3 抗体

This anti-Plakophilin 3 antibody is a 小鼠 单克隆 antibody detecting Plakophilin 3 in WB, ELISA, IHC, IP, IF 和 IHC (p). Suitable for 人, 小鼠 和 犬.
产品编号 ABIN201418
发货至: 中国

Quick Overview for Plakophilin 3 抗体 (ABIN201418)

抗原

See all Plakophilin 3 (PKP3) 抗体
Plakophilin 3 (PKP3)

适用

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人, 小鼠, 犬

宿主

  • 16
  • 13
  • 2
小鼠

克隆类型

  • 17
  • 14
单克隆

标记

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This Plakophilin 3 antibody is un-conjugated

应用范围

  • 31
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  • 5
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  • 2
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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 特异性

    Reacts with the 87kD human Plakophilin-3. There is no cross reactivity with human Plakophilin-1 and Plakophilin-2 proteins. Reactivity is confirmed with human A431 cells, and HEK293 cells transfected with plasmid expressing human Plakophilin-3. Based on sequence homology, this antibody is expected to react with mouse and canine.

    纯化方法

    Protein A purified

    免疫原

    Synthetic peptide from Armadillo domain of human Plakophilin-3.

    Type of Immunogen: Synthetic peptide

    亚型

    IgG2b
  • 应用备注

    Approved: ELISA (0.1 - 1 μg/mL), IF (1 - 3 μg/mL), IHC, IHC-P, IP, WB (1 - 3 μg/mL)

    Usage: Suitable for use in ELISA, Immunoprecipitation (native), Immunohistochemistry (formalin fixed paraffin embedded), Immunofluorescence and Western Blot. ELISA: 0.1-1 μg/mL. Immunoprecipitation: 10 μg/IP reaction. Immunohistochemistry: 10 μg/mL. Immunofluorescence: 1-3 μg/mL. Western Blot: 1-3 μg/mL. Note: Immunofluorescent assays were performed using methanol-fixed HCT8/E8 and paraformaldehyde-fixed HaCat cells. For optimal IF results, methanol and paraformaldehyde- fixed cells should be permeabilized with 0.2 % Triton X-100 for 5 and 15 minutes, respectively, prior to incubation with primary antibody.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.1 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • 抗原

    Plakophilin 3 (PKP3)

    别名

    PKP3 / Plakophilin 3

    背景

    Name/Gene ID: PKP3

    Synonyms: PKP3, Plakophilin 3, Plakophilin-3, Plakophilin 3b

    基因ID

    11187

    UniProt

    Q9Y446
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