ITGAV/ITGB6 抗体
The 小鼠 单克隆 anti-ITGAV/ITGB6 antibody is suitable to detect ITGAV/ITGB6 in samples from 人, 小鼠 和 大鼠. It has been validated for FACS, IP 和 Func.
产品编号 ABIN201125
发货至:
中国
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Quick Overview for ITGAV/ITGB6 抗体 (ABIN201125)
抗原
ITGAV/ITGB6
(Integrin alpha V, Integrin beta 6 (ITGAV/ITGB6))
适用
人, 小鼠, 大鼠
宿主
小鼠
克隆类型
单克隆
标记
This ITGAV/ITGB6 antibody is un-conjugated
应用范围
Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)
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特异性
- Recognizes mouse integrin alpha V beta 6. Blocks activity against mouse and human alpha V beta 6. Does not recognize alpha V or other alpha V integrins. Species cross-reactivity: Human, rat and mouse. Because the antibody was raised in knockout mice, it is likely to recognize the receptor in other species.
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纯化方法
- Protein A purified
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免疫原
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Immunization of beta 6 knockout mice with wild type murine keratinocytes.
Type of Immunogen: Cells -
亚型
- IgG2a
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anti-Integrin alpha V, Integrin beta 6 (ITGAV/ITGB6) antibody
ITGAV/ITGB6 适用: 人, 小鼠, 大鼠 FACS, WB, IF (cc), IF (p), ELISA, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: Flo, Func, IP
Usage: Suitable for use in Flow Cytometry, Immunoprecipitation and Function Blocking. Not suitable for use in Western Blot and Immunohistochemistry. Flow Cytometry (effective for mouse and human): Use buffers containing 1 mM Mg, since the epitope recognized is divalent cation-dependent. Immunoprecipitation: Effective. Protein G or rabbit anti-mouse IgG recommended for capture. Function blocking: Inhibition of alpha V beta 6-mediated cell adhesion and cell migration are achieved at a concentration of 100 μg/mL using beta 6 transfected cells tested against TGFbeta LAP. Note: Cell lines adhere to simple ligands (e. g. fibronectin) using more integrins than alphav/beta6, so it would be necessary to also block other integrins (e. g. with anti-beta1 antibody) to see any effect of this antibody on adhesion to fibronectin. For example, H441 cells, which naturally express high levels of alphaV/beta6, may be used as a positive control.
Not recommended for: IHC, WB -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS. No preservatives added.
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储存液
- Without preservative
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- ITGAV/ITGB6 (Integrin alpha V, Integrin beta 6 (ITGAV/ITGB6))
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别名
- Integrin AlphaV Beta6
抗原
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