ITGA6 抗体
Quick Overview for ITGA6 抗体 (ABIN201115)
抗原
See all ITGA6 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Reactive with chicken integrin alpha6, as demonstrated by experiments with cDNA-transfected NIH3T3 cells followed by immunoblots.
-
纯化方法
- Purified
-
免疫原
- Integrin alpha subunits purified from adult chicken brain tissue, denatured in 6 M guanidine isothiocyanate, and depleted of beta1 subunit.
-
亚型
- IgG
-
-
-
-
应用备注
-
Approved: ICC, IHC, IP, WB
Usage: Western Blot. Immunoprecipitation: Precipitates the monomeric alpha6 subunit after the dimeric receptor has been denatured, but does not precipitate the native receptor. Immunohistochemistry: Methanol-fixed cryosections. An overnight methanol fix is suggested. Previous experiments have used 14 micron sections of chicken embryonic tissue. Typical antibodies dilutions are 35 μg/mL, with overnight antibody incubation, followed by detection with a goat anti-mouse IgG-FITC secondary antibody. Immunocytochemistry: Methanol-fixed cultured cells (15 minute fixation). Western Blot: Recognizes a 130kD band in chicken brain tissue and a 140kD band in transfected NIH3T3 cells (the difference in molecular weight is likely due to differential glycosylation). -
说明
-
Target Species of Antibody: Chicken
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, pH 7.6, 0.1 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- avoid freeze thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
-
-
- ITGA6 (Integrin, alpha 6 (ITGA6))
-
别名
- ITGA6/Integrin Alpha 6/CD49f
-
背景
-
Name/Gene ID: ITGA6
Family: Integrin
Synonyms: ITGA6, CD49f, CD49f antigen, Integrin alpha6B, Integrin, alpha 6, Integrin alpha-6 precursor, VLA-6, Integrin alpha-6, ITGA6B -
基因ID
- 3655
-
UniProt
- P23229
-
途径
- CXCR4-mediated Signaling Events, Brown Fat Cell Differentiation, Integrin Complex
抗原
-