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ITGA6 抗体

This anti-ITGA6 antibody is a 小鼠 单克隆 antibody detecting ITGA6 in WB, IHC, IP 和 ICC. Suitable for 小鸡.
产品编号 ABIN201115
发货至: 中国

Quick Overview for ITGA6 抗体 (ABIN201115)

抗原

See all ITGA6 抗体
ITGA6 (Integrin, alpha 6 (ITGA6))

适用

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小鸡

宿主

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小鼠

克隆类型

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单克隆

标记

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This ITGA6 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • 特异性

    Reactive with chicken integrin alpha6, as demonstrated by experiments with cDNA-transfected NIH3T3 cells followed by immunoblots.

    纯化方法

    Purified

    免疫原

    Integrin alpha subunits purified from adult chicken brain tissue, denatured in 6 M guanidine isothiocyanate, and depleted of beta1 subunit.

    亚型

    IgG
  • 应用备注

    Approved: ICC, IHC, IP, WB

    Usage: Western Blot. Immunoprecipitation: Precipitates the monomeric alpha6 subunit after the dimeric receptor has been denatured, but does not precipitate the native receptor. Immunohistochemistry: Methanol-fixed cryosections. An overnight methanol fix is suggested. Previous experiments have used 14 micron sections of chicken embryonic tissue. Typical antibodies dilutions are 35 μg/mL, with overnight antibody incubation, followed by detection with a goat anti-mouse IgG-FITC secondary antibody. Immunocytochemistry: Methanol-fixed cultured cells (15 minute fixation). Western Blot: Recognizes a 130kD band in chicken brain tissue and a 140kD band in transfected NIH3T3 cells (the difference in molecular weight is likely due to differential glycosylation).

    说明

    Target Species of Antibody: Chicken

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.6, 0.1 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    avoid freeze thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • 抗原

    ITGA6 (Integrin, alpha 6 (ITGA6))

    别名

    ITGA6/Integrin Alpha 6/CD49f

    背景

    Name/Gene ID: ITGA6
    Family: Integrin

    Synonyms: ITGA6, CD49f, CD49f antigen, Integrin alpha6B, Integrin, alpha 6, Integrin alpha-6 precursor, VLA-6, Integrin alpha-6, ITGA6B

    基因ID

    3655

    UniProt

    P23229

    途径

    CXCR4-mediated Signaling Events, Brown Fat Cell Differentiation, Integrin Complex
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