GJA3 抗体 (Internal Region)
Quick Overview for GJA3 抗体 (Internal Region) (ABIN200660)
抗原
See all GJA3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- Recognizes mouse Connexin 46 protein. Species sequence homology: rat, Chinese hamster and Golden Hamster.
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide derived from an internal region of the mouse Connexin 46 protein.
Type of Immunogen: Synthetic peptide
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应用备注
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Approved: IF (3 μg/mL), IHC, IHC-Fr, WB (1 - 3 μg/mL)
Usage: Suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence. Immunohistochemistry (Frozen): 3 μg/mL. Western Blot: 1-3 μg/mL. Identifies a single band at ~46kD. Immunofluorescence: 3 μg/mL. Note: For IHC, mice were perfused transcardially with 3 mL pre-fixative (50 mM phosphate buffer, 0.1 % sodium nitrite, 1 unit/mL heparin), followed by 40 mL fixative (0.16M sodium phosphate buffer, pH 7.6, 0.2 % picric acid, and 1-, 2-, or 4 % paraformaldehyde), and perfusion with 10 mL sucrose wash (10 % sucrose in 25 mM sodium phosphate buffer, pH 7.4. Tissues were then stored in cryoprotectant (10 % sucrose, 25 mM phosphate buffer, pH 7.4, 0.04 % sodium azide) for a minimum of 24 hours prior to sectioning. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- GJA3 (Gap Junction Protein, alpha 3, 46kDa (GJA3))
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别名
- GJA3 / CX46 / Connexin 46
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背景
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Name/Gene ID: GJA3
Subfamily: Connexin
Family: Ion Channel
Synonyms: GJA3, Connexin-46, CX46, CZP3, Gap junction alpha-3 protein, Gap junction protein alpha 3, CAE3, Connexin 46 -
基因ID
- 2700
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UniProt
- Q9Y6H8
抗原
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