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CD51/CD61 抗体

This anti-CD51/CD61 antibody is a 小鼠 单克隆 antibody detecting CD51/CD61 in FACS, IP, IHC (fro), IF, IHC 和 ICC. Suitable for 人, 小鸡, 兔, Cow, 犬, 猴, Pig 和 Avian.
产品编号 ABIN199326
发货至: 中国

Quick Overview for CD51/CD61 抗体 (ABIN199326)

抗原

See all CD51/CD61 (ITGAV/ITGB3) 抗体
CD51/CD61 (ITGAV/ITGB3) (Integrin alpha V, Integrin beta 3 (ITGAV/ITGB3))

适用

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人, 小鸡, 兔, Cow, 犬, 猴, Pig, Avian

宿主

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小鼠

克隆类型

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单克隆

标记

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This CD51/CD61 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

克隆位点

LM609
  • 特异性

    Recognizes the human vitronectin receptor aVb3 complex, an RGD-directed adhesion receptor. Species cross-reactivity: bovine, chicken, monkey, porcine, canine, rabbit and avian. Not reactive with rat tissue.

    纯化方法

    Protein A purified

    免疫原

    Purified adhesion receptor from the human melanoma cell line M21.

    Type of Immunogen: Cells

    亚型

    IgG1
  • 应用备注

    Approved: Flo, ICC, IF (5 - 10 μg/mL), IHC, IHC-Fr, IP

    Usage: Suitable for use in Immunoprecipitation, Immunofluorescence, Flow Cytometry, Immunohistochemistry (frozen sections only), Immunocytochemistry and Blocking. Immunoprecipitation: Precipitates integrin alpha V beta3 complex. When resolved by SDS-PAGE under reducing conditions, bands are observed at ~130kD corresponding to the alpha V subunit and at ~105kD corresponding to the beta3 subunit. Flow Cytometry: 1-3 μg/0.5x1E6cells in 100 μL volume. Immunofluorescence: 5-10 μg/mL for staining of bFGF-treated 6mm cryosections of chick chorioallantoic membrane fixed with acetone. Also effective on 4 % paraformaldehyde fixed, frozen tissues and cells. Not effective for immunohistochemical staining of paraffin-embedded tissue sections. Blocking: Inhibits adhesion of cells to vitronectin coated surfaces at 10-25 μg/mL. Positive control: Human myeloma cell lines M21 and MoalphaV.

    Not recommended for: IHC-P

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.1. No preservatives added.

    储存液

    Without preservative

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    CD51/CD61 (ITGAV/ITGB3) (Integrin alpha V, Integrin beta 3 (ITGAV/ITGB3))

    别名

    Integrin Alpha V Beta 3
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