Cx40/GJA5 抗体 (C-Term)
Quick Overview for Cx40/GJA5 抗体 (C-Term) (ABIN199015)
抗原
See all Cx40/GJA5 (GJA5) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- Reacts with the mouse Connexin 40 protein. On Western Blots, it identifies the target band at ~40kD. Reactivity has been confirmed with mouse heart homogenates by Western Blot, mouse heart and lung homogenates by immunoprecipitation, and mouse heart tissue by immunohistochemistry and immunofluorescence. Species cross-reactivity: Cross-reactivity with rat and human CX40 proteins is expected based on amino acid sequence homology.
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide derived from the C-terminal region of the mouse and rat Connexin 40 (CX40) proteins. The human sequence at this location differs by only one amino acid.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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anti-Gap Junction Protein, alpha 5, 40kDa (GJA5) (AA 301-358) antibody
GJA5 适用: 人, 大鼠, 小鼠, 犬 WB, ELISA, IHC (p), IHC (fro), FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 5, 40kDa (GJA5) (AA 227-358) antibodyGJA5 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 5, 40kDa (GJA5) (AA 86-135) antibodyGJA5 适用: 人, 犬, Cow, 马 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 5, 40kDa (GJA5) (N-Term) antibodyGJA5 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 5, 40kDa (GJA5) (N-Term) antibodyGJA5 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 5, 40kDa (GJA5) (C-Term) antibodyGJA5 适用: 人, 小鼠 WB, ELISA, IHC, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal 2F9A11 unconjugated
anti-Gap Junction Protein, alpha 5, 40kDa (GJA5) (AA 229-358) antibodyGJA5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 5, 40kDa (GJA5) (AA 50-303) antibodyGJA5 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 5, 40kDa (GJA5) antibodyGJA5 适用: 人 WB, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Gap Junction Protein, alpha 5, 40kDa (GJA5) (N-Term) antibodyGJA5 适用: 人 WB, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: IF (1 - 3 μg/mL), IHC, IHC-Fr, IP, WB (1 - 3 μg/mL)
Usage: Suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Western Blot: 1-3 μg/mL. Immunofluorescence: 1-3 μg/mL. Immunoprecipitation: 7 μg/reaction. Immunohistochemistry (Frozen sections): 1-3 μg/mL, For IHC, mice were perfused transcardially with 3 mL pre-fixative (50 mM phosphate buffer, 0.1 % sodium nitrite, 1 unit/mL heparin),. followed by 40 mL fixative (0.16M sodium phosphate buffer, 0.2 % picric acid, and 1-, 2-, or 4 % paraformaldehyde), and perfusion with 10 mL sucrose wash (10 % sucrose in 25 mM sodium phosphate buffer, pH 7.4). Tissues were then stored in cryoprotectant (10 % sucrose, 25 mM phosphate buffer, pH 7.4, 0.04 % sodium azide) for a minimum of 24 hours prior to sectioning. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- Cx40/GJA5 (GJA5) (Gap Junction Protein, alpha 5, 40kDa (GJA5))
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别名
- GJA5 / CX40 / Connexin 40
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背景
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Name/Gene ID: GJA5
Subfamily: Connexin
Family: Ion Channel
Synonyms: GJA5, Connexin 40, Connexin-40, Gap junction alpha-5 protein, ATFB11, CX40, Gap junction protein alpha 5 -
基因ID
- 2702
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UniProt
- P36382
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途径
- Cell-Cell Junction Organization
抗原
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