VASP 抗体 (pSer239)
Quick Overview for VASP 抗体 (pSer239) (ABIN198238)
抗原
See all VASP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 236-242, pSer239
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特异性
- Recognizes human phosphorylated VASP, Mr 50kD. Species cross-reactivity: mouse.
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纯化方法
- Gel filtration
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免疫原
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KLH-conjugated, synthetic phosphopeptide corresponding to amino acids 236-242 of human phospho-VASP (RKV[pS]KQE).
Type of Immunogen: Synthetic phospho-peptide -
亚型
- IgG1 kappa
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应用备注
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Approved: ELISA (0.05 μg/mL), Flo (2.4 μg/mL), ICC, IP, WB
Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry, FACS and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: An independent laboratory has reported that 3 μg/mL antibody showed positive immunostaining in SNP or 8pCPT-cGMP-treated human platelets, fixed with 4 % paraformaldehyde and permeabilized with 0.2 % Triton-X. FACS: 2.4 μg/mL using fixed and permeabilized human platelets, as reported by an independent laboratory. Immunoprecipitation: Immunoprecipitates phosphorylated VASP from vanadate-treated A431 cells, as reported by an independent laboratory. ELISA: 0.05 μg/mL, as reported by an independent laboratory. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 1 mL ddH2O
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浓度
- Lot specific
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缓冲液
- Supplied as a lyophilized powder from PBS, 0.09 % sodium azide, PEG, sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Avoid freeze-thaw cycles.
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- VASP (Vasodilator-Stimulated phosphoprotein (VASP))
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别名
- VASP
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背景
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Name/Gene ID: VASP
Family: Ena-VASP
Synonyms: VASP -
基因ID
- 7408
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UniProt
- P50552
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途径
- TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation
抗原
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