HDAC4 抗体 (Ser632)
Quick Overview for HDAC4 抗体 (Ser632) (ABIN197338)
抗原
See all HDAC4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Ser632
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特异性
- This antibody AP02668PU detects endogenous levels of total HDAC4 protein.
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纯化方法
- Immunoaffinity Chromatography using epitope-specific immunogen.
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免疫原
- The antiserum was produced against synthesized non-phosphopeptide derived from Human HDAC4 around the phosphorylation site of Serine 632 (A-Q-Sp-S-P).
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anti-Histone Deacetylase 4 (HDAC4) (AA 904-1076) antibody
HDAC4 适用: 小鼠 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 551-648) antibodyHDAC4 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2B11 unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 456-592) antibodyHDAC4 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 7E2E6 unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 456-592) antibodyHDAC4 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 5E4A5 unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 451-650) antibodyHDAC4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 1049-1077) antibodyHDAC4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) antibodyHDAC4 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) (pSer632) antibodyHDAC4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 530-630) antibodyHDAC4 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) antibodyHDAC4 适用: 人, 小鼠, 大鼠 WB, IF (cc) 宿主: 兔 Monoclonal 6B10 unconjugated
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应用备注
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Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at -20 °C.
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- HDAC4 (Histone Deacetylase 4 (HDAC4))
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别名
- HDAC4
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背景
- Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p. HDAC4 is a class II histone deacetylase containing 1084 amino acid residues. HDAC4 has been shown to interact with NCoR. HDAC4 is a member of the class II mammalian histone deacetylases, which consists of 1084 amino acid residues. Its C terminal sequence is highly similar to the deacetylase domain of yeast HDA1. HDAC4, unlike other deacetylases, shuttles between the nucleus and cytoplasm in a process involving active nuclear export. Association of HDAC4 with 14-3-3 results in sequestration of HDAC 4 protein in the cytoplasm. In the nucleus, HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART.Synonyms: HD4, Histone deacetylase 4, KIAA0288
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基因ID
- 9759
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NCBI登录号
- NP_006028
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UniProt
- P56524
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process
抗原
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