Histone H3.1 抗体 (Ser10)
Quick Overview for Histone H3.1 抗体 (Ser10) (ABIN197332)
抗原
See all Histone H3.1 (HIST1H3B) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Ser10
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特异性
- This antibody detects endogenous levels of total Histone H3.1 protein.
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纯化方法
- Immunoaffinity Chromatography using epitope-specific immunogen.
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免疫原
- The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H3.1 around the phosphorylation site of Serine 10 (R-K-Sp-T-G).
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anti-Histone H3.1 (HIST1H3B) (pSer10) antibody
HIST1H3B 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histone H3.1 (HIST1H3B) (pSer28) antibodyHIST1H3B 适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 5D10D4 unconjugated
anti-Histone H3.1 (HIST1H3B) (AA 21-39) antibodyHIST1H3B 适用: 人 WB, IF, ChIP, ICC, ChIP-seq 宿主: 小鼠 Monoclonal 1D4F2 unconjugated
anti-Histone H3.1 (HIST1H3B) (pSer28), (Ser28) antibodyHIST1H3B 适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 5D10D4 unconjugated
anti-Histone H3.1 (HIST1H3B) (AA 1-50), (pSer10) antibodyHIST1H3B 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Histone H3.1 (HIST1H3B) (pSer28) antibodyHIST1H3B 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at -20 °C.
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- Histone H3.1 (HIST1H3B)
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别名
- Histone H3.1
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背景
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways.Synonyms: H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, H3FA, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
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基因ID
- 8350
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NCBI登录号
- NP_003520
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UniProt
- P68431
抗原
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