ZAP70 抗体 (pTyr319)
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Quick Overview for ZAP70 抗体 (pTyr319) (ABIN196884)
抗原
See all ZAP70 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr319
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特异性
- This antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyrosine 319.
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纯化方法
- Immunoaffinity Chromatography
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免疫原
- Synthetic phosphopeptide derived from human Zap-70 around the phosphorylation site of Tyrosine 319 (S-P-Yp-S-D).
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应用备注
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Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at -20 °C.
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- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
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别名
- ZAP70
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背景
- Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70
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基因ID
- 7535
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NCBI登录号
- NP_001070
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UniProt
- P43403
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途径
- TCR Signaling, Ubiquitin Proteasome Pathway
抗原
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