NCOA1 抗体 (pTyr529)
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Quick Overview for NCOA1 抗体 (pTyr529) (ABIN196876)
抗原
See all NCOA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr529
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特异性
- The antibody detects endogenous levels of Src only when phosphorylated at Tyrosine 529.
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纯化方法
- Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- The antiserum was produced against synthesized phosphopeptide derived from human Src around the phosphorylation site of tyrosine 529 (P-Q-YP-Q-P).
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应用备注
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Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at -20 °C.
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- NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))
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别名
- SRC1
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背景
- Synonyms: Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, c-Src, pp60c-src
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基因ID
- 6714
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NCBI登录号
- NP_005408
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UniProt
- P12931
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha
抗原
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