LCK 抗体 (pTyr394)
-
- 抗原 See all LCK 抗体
- LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
-
抗原表位
- pTyr394
-
适用
- 人, 小鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This LCK antibody is un-conjugated
-
应用范围
- Western Blotting (WB)
- 特异性
- This antibody detects endogenous levels of Lck only when phosphorylated at Tyrosine 394.
- 交叉反应 （详细）
- Species reactivity (tested):Human, Mouse.
- 纯化方法
- Immunoaffinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- 免疫原
- Peptide sequence around the phosphorylation site of Tyrosine 394 (N-E-pY-T-A) derived from Human Lck.
- Top Product
- Discover our top product LCK Primary Antibody
-
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) antibody
LCK 适用: 人 WB, ELISA, IP, RNAi 宿主: 小鼠 Monoclonal 3F7-F5 unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-240) antibodyLCK 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 22-36) antibodyLCK 适用: 人 WB, FACS, IP, ICC 宿主: 小鼠 Monoclonal LCK-01 unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 23-52) antibodyLCK 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) antibodyLCK 适用: 人 ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 468-506), (C-Term) antibodyLCK 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 480-509) antibodyLCK 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 421-509) antibodyLCK 适用: 人, 小鼠 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) antibodyLCK 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-100) antibodyLCK 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3H5 unconjugated
-
- 应用备注
-
Suitable for use in Western blot (1: 500approx. 1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody (in aliquots) at -20 °C.
-
- 抗原
- LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
- 别名
- LCK (LCK 产品)
- 别名
-
zgc:136695 antibody, LCK antibody, Hck-3 antibody, Lsk antibody, Lskt antibody, p56
antibody, p56Lck antibody, LSK antibody, YT16 antibody, p56lck antibody, pp58lck antibody, P56LCK antibody, tkl antibody, Lck1 antibody, Lcktkr antibody, LCK proto-oncogene, Src family tyrosine kinase antibody, lymphocyte protein tyrosine kinase antibody, lck antibody, LCK antibody, Lck antibody - 背景
- Lck is a lymphoid specific cytosolic protein tyrosine kinase (PTK), which is essential for T cell development and function. It is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T cell antigen receptor (TCR) linked signal transduction pathways. Association of the TCR with a peptide antigen bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated Lck to the vicinity of the TCR/CD3 complex. Lck then phosphorylates tyrosine residues within the immunoreceptor tyrosine based activation motifs (ITAMs) in the cytoplasmic tails of the TCR chains and CD3 subunits. The phosphoITAMs serve as docking sites for Src homology domain 2 (SH2) containing molecules, predominantly ZAP 70 and Syk. Only then can ZAP 70 undergo tyrosine phosphorylation, become enzymatically active and further phosphorylate downstream effector molecules. In addition, Lck contributes to signaling by other receptor molecules. Lck is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature TCR.Synonyms: LSK, Lymphocyte cell-specific protein-tyrosine kinase, Proto-oncogene tyrosine-protein kinase LCK, T cell-specific protein-tyrosine kinase, p56-LCK
- 分子量
- 56 kDa
- 基因ID
- 3932
- NCBI登录号
- NP_001036236
- UniProt
- P06239
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
-
