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Pyruvate Kinase, Muscle (PKM) 抗体 (Biotin)

The Biotin-conjugated 山羊 多克隆 anti-Pyruvate Kinase, Muscle antibody (ABIN1968075) specifically detects Pyruvate Kinase, Muscle in WB 和 ELISA. The antibody is reactive with 兔 samples.
产品编号 ABIN1968075
发货至: 中国
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Quick Overview for Pyruvate Kinase, Muscle (PKM) 抗体 (Biotin) (ABIN1968075)

抗原

See all Pyruvate Kinase, Muscle (PKM) 抗体
Pyruvate Kinase, Muscle (PKM)

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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Biotin

应用范围

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Western Blotting (WB), ELISA
  • 特异性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Anti-Pyruvate Kinase has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other sources may occur but have not been specifically determined.

    纯化方法

    Ion exchange chromatography

    免疫原

    Pyruvate Kinase [Rabbit Muscle].

    Type of Immunogen: Purified protein
  • 应用备注

    Approved: ELISA (1:4000 - 1:20000), WB (1:500 - 1:2000)

    Usage: Anti-Pyruvate Kinase Antibody has been assayed against 1.0 μg of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:15000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Rabbit

    限制

    仅限研究用
  • 状态

    Liquid

    溶解方式

    Distilled water

    浓度

    Lot specific

    缓冲液

    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA

    注意事项

    avoid freeze thaw cycles

    储存条件

    4 °C,-20 °C

    储存方法

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
  • 抗原

    Pyruvate Kinase, Muscle (PKM)

    别名

    PKM / Pyruvate Kinase, Muscle

    背景

    Name/Gene ID: PKM

    Synonyms: PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kinase 2/3, Pyruvate kinase, m1, Pyruvate kinase, muscle, TCB, Tumor M2-PK, OIP-3, OIP3, Opa-interacting protein 3, CTHBP, PK, muscle type, PK2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, THBP1

    基因ID

    5315

    途径

    Warburg Effect
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