Pyruvate Kinase, Muscle (PKM) 抗体 (Biotin)
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Quick Overview for Pyruvate Kinase, Muscle (PKM) 抗体 (Biotin) (ABIN1968075)
抗原
See all Pyruvate Kinase, Muscle (PKM) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Anti-Pyruvate Kinase has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other sources may occur but have not been specifically determined.
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纯化方法
- Ion exchange chromatography
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免疫原
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Pyruvate Kinase [Rabbit Muscle].
Type of Immunogen: Purified protein
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应用备注
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Approved: ELISA (1:4000 - 1:20000), WB (1:500 - 1:2000)
Usage: Anti-Pyruvate Kinase Antibody has been assayed against 1.0 μg of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:15000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Rabbit
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Distilled water
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浓度
- Lot specific
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缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA
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注意事项
- avoid freeze thaw cycles
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
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- Pyruvate Kinase, Muscle (PKM)
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别名
- PKM / Pyruvate Kinase, Muscle
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背景
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Name/Gene ID: PKM
Synonyms: PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kinase 2/3, Pyruvate kinase, m1, Pyruvate kinase, muscle, TCB, Tumor M2-PK, OIP-3, OIP3, Opa-interacting protein 3, CTHBP, PK, muscle type, PK2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, THBP1 -
基因ID
- 5315
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途径
- Warburg Effect
抗原
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