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Caveolin-1 抗体 (pTyr14)

The 兔 多克隆 anti-Caveolin-1 antibody is suitable to detect Caveolin-1 in samples from 人, 大鼠 和 小鼠. It has been validated for WB 和 IF.
产品编号 ABIN196778
发货至: 中国
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Quick Overview for Caveolin-1 抗体 (pTyr14) (ABIN196778)

抗原

See all Caveolin-1 (CAV1) 抗体
Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

适用

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人, 大鼠, 小鼠

宿主

  • 138
  • 11
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  • 2

克隆类型

  • 100
  • 57
多克隆

标记

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This Caveolin-1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF)
  • 抗原表位

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    pTyr14

    特异性

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Antibody AP02388PU detects endogenous levels of Caveolin-1 only when phosphorylated at Tyrosine 14.

    纯化方法

    Immunoaffinity Chromatography.

    免疫原

    The antiserum was produced against synthesized phosphopeptide derived from human Caveolin-1 around the phosphorylation site of tyrosine 14 (H-L-YP-T-V).
  • 应用备注

    Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store the antibody (in aliquots) at -20 °C.
  • 抗原

    Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

    别名

    Caveolin-1

    背景

    Caveolae (also known as plasmalemmal vesicles) are 50-100 nm flask-shaped membranes that represent a subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules (including LDL) across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules including G protein-coupled receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3 have been identified as principle components of caveolae.Synonyms: CAV1

    基因ID

    857

    NCBI登录号

    NP_001744

    UniProt

    Q03135

    途径

    Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals
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