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CXCR3 抗体

This anti-CXCR3 antibody is a 小鼠 单克隆 antibody detecting CXCR3 in FACS, IHC, Neut 和 Func. Suitable for 人.
产品编号 ABIN1967162
发货至: 中国

Quick Overview for CXCR3 抗体 (ABIN1967162)

抗原

See all CXCR3 抗体
CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CXCR3 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Neutralization (Neut), Functional Studies (Func)

克隆位点

2AR1
  • 特异性

    Recognizes human CXCR3. Reacts specifically with CXCR3 transfectants and not the parental cell line using FACS analysis. Does not cross-react with CXCR1, CXCR2, CXCR4 or transfectants.

    纯化方法

    Protein G purified

    免疫原

    CXCR3 transfected NS0 cells.

    Type of Immunogen: Cells

    亚型

    IgG1
  • 应用备注

    Approved: Flo (5 - 10 μg/mL), Func, IHC (10 μg/mL), Neut

    Usage: Suitable for use in Neutralization, Flow Cytometry and Immunohistochemistry. Neutralization (ND50): The exact concentration required to neutralize human cell surface CXCR3 activity is dependent on the ligand concentration as well as the number of CXCR3 receptors present on the cell surface. The Neutralization Dose50 (ND50) is defined as the concentration of antibody required to yield one half maximal inhibition of the cell surface CXCR3 mediated rhl-TAC response on a responsive cell line, at a specific rhl-TAC concentration. The ND50 for this lot was determined to be ~1-6 μg/mL in the presence of 7 ng/mL of rhl-TAC, using the hCXCR3 transfected BaF/3 cells in a chemotaxis assay. Flow Cytometry: 5-10 μg/mL, Add 10 μL to 1-2.5x10^5 cells in total reaction volume 200 μL. Visualize binding by adding 10 μL of 25 μg/mL IgG (FITC) goat anti-mouse. Immunohistochemistry: 10 μg/mL detected CXCR3 in human tonsil. Cells were fixed in PBS, 4 % paraformaldehyde and blocked with PBS, 10 % normal donkey serum, 0.1 % Triton X-100, 1 % BSA. Cells were washed with PBS, BSA.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    200 μL sterile PBS

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS, 5 % trehalose.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.
  • 抗原

    CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

    别名

    CXCR3

    背景

    Name/Gene ID: CXCR3
    Subfamily: Chemokine
    Family: GPCR

    Synonyms: CXCR3, C-x-c chemokine receptor 3, CD183 antigen, CKR-L2, CXCR-3, CMKAR3, CD182, CD183, Cxcr3a, GPR9, IP-10 receptor, IP10-R, Mig receptor, MigR, Mig-R, Chemokine (C-X-C) receptor 3, CXC-R3, Cxcr3b, G protein-coupled receptor 9, IP10 receptor

    基因ID

    2833

    UniProt

    P49682
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