Proliferation Marker 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in IHC, WB, ELISA, IHC (fro), IHC (p) 和 IP. Suitable for 人.
Quick Overview for Proliferation Marker 抗体 (ABIN1959948)
抗原
Proliferation Marker
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
克隆位点
IPO-38
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品牌
- IHC-plus™
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特异性
- A 14 -16 kD protein as demonstrated by western blot analysis on the Raji cell line. This antigen was detected after 12 h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes. Cross reactivity is likely to occur with mouse and rat. Reactivity with other sources has not been determined.
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纯化方法
- Protein A purified
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免疫原
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Spleen cells of a human patient with hairy cell leukemia.
Type of Immunogen: Cells
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anti-Proliferation Marker antibody
适用: 人 IHC, WB, IF, StM 宿主: 小鼠 Monoclonal JC1 unconjugated
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应用备注
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Approved: ELISA (1:10000 - 1:50000), IHC, IHC-Fr, IHC-P (10 μg/mL), IP, WB (1:500 - 1:2000)
Usage: This antibody is suitable for ELISA, immunoprecipitation, western blotting and immunohistochemistry (frozen and formal in/paraffin). The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-1 00 permeabilized). An increase of 400 % is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemide). IPO-38 does not block the binding of the Ki-67 antibody. Tonsilar tissue is typically used as a positive control. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
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Short term: -20°C
Long term: -20°C.
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- Proliferation Marker
抗原
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