GLB1 抗体 (HRP)
Quick Overview for GLB1 抗体 (HRP) (ABIN1952606)
抗原
See all GLB1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, and anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase [E.coli]. Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.
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纯化方法
- Ion exchange chromatography
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免疫原
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Beta Galactosidase (E. coli).
Type of Immunogen: Purified protein
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anti-Galactosidase, beta 1 (GLB1) antibody
GLB1 适用: 人, 犬, 猴 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1C9 unconjugated
anti-Galactosidase, beta 1 (GLB1) (AA 1-270) antibodyGLB1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Galactosidase, beta 1 (GLB1) (AA 68-219) antibodyGLB1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Galactosidase, beta 1 (GLB1) (AA 578-677) antibodyGLB1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 6E7 unconjugated
anti-Galactosidase, beta 1 (GLB1) (AA 317-455) antibodyGLB1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Galactosidase, beta 1 (GLB1) antibodyGLB1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Galactosidase, beta 1 (GLB1) (AA 192-432) antibodyGLB1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Galactosidase, beta 1 (GLB1) (AA 301-380) antibodyGLB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Galactosidase, beta 1 (GLB1) antibodyGLB1 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Galactosidase, beta 1 (GLB1) (AA 1-677) antibodyGLB1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: DB, ELISA (1:10000 - 1:50000), IF, IHC (1:500 - 1:2000), IP, WB (1:2000 - 1:5000)
Usage: Anti-Beta Galactosidase is suitable for immunoblotting (western or dot blot), ELISA, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4 % paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:5000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus. A 1:1 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4 % paraformaldehyde and cryostat-cut (35 mu m) to produce free-floating sections. A 1:5000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy. A 1:5000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos. Although a wide range of conditions was reported to be effective, a 1:10000 dilution was noted to show no background and to be suitable for double labeling experiments. Optimal titers for other applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: E. coli
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Distilled water
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浓度
- Lot specific
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缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % Gentamicin sulfate.
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储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
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- GLB1 (Galactosidase, beta 1 (GLB1))
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别名
- Beta Galactosidase
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途径
- Glycosaminoglycan Metabolic Process
抗原
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