SOX2 抗体 (N-Term)
Quick Overview for SOX2 抗体 (N-Term) (ABIN1937403)
抗原
See all SOX2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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特异性
- Human SOX2
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纯化方法
- Protein A/G purified
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免疫原
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Synthetic peptide corresponding to N-terminus of human SOX-2 protein.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: IHC, IHC-P (1:100), WB (1:25)
Usage: IMMUNOHISTOCHEMISTRY: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. Antigen Retrieval: Boil tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween. IHC Positive control: Newborn brain. WESTERN BLOT: Recommended starting protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate. actual results may differ because of variability in methods and protocols. Positive Control: MCF7 cell lysate. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.6, 1 % BSA, 0.1 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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- SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))
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别名
- SOX2
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背景
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Name/Gene ID: SOX2
Family: Transcription factor
Synonyms: SOX2, MCOPS3, Transcription factor SOX2, ANOP3, Transcription factor SOX-2, SRY-related HMG-box gene 2 -
基因ID
- 6657
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途径
- Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis
抗原
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