Alpha, beta-Tubulin Dimer 抗体
适用: Pig, 人, 小鼠
WB, ICC
宿主: 小鼠
Monoclonal
TU-08
unconjugated
1 image
(1 reference)-
- 抗原
- Alpha, beta-Tubulin Dimer
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适用
- Pig, 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunocytochemistry (ICC)
- 特异性
- The antibody TU-08 recognizes alpha, beta-tubulin heterodimer (porcine brain), a basic intracellular structural unit of microtubules. Alpha- and beta- tubulins form approximately 100 kDa tubulin heterodimer, a globular protein that polymerizes to form microtubules.
- 交叉反应 （详细）
- Human, Porcine, Mouse, Other not tested
- 纯化方法
- Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
- 纯度
- > 95 %
- 免疫原
- Microtubule proteins from porcine brain.
- 克隆位点
- TU-08
- 亚型
- IgM
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anti-Alpha, beta-Tubulin Dimer antibody
适用: Pig, 人, 小鼠, Non-Human Primate, 大鼠 WB, ICC, FACS 宿主: 小鼠 Monoclonal TU-10 unconjugated
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- 应用备注
- Western blotting: Recommended dilution: 2 μg/mL, positive control: porcine brain lysate.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "Novel monoclonal antibodies TU-08 and TU-16 specific for tubulin subunits." in: Folia biologica, Vol. 44, Issue 1, pp. 35-6, (2000) (PubMed).
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: "Novel monoclonal antibodies TU-08 and TU-16 specific for tubulin subunits." in: Folia biologica, Vol. 44, Issue 1, pp. 35-6, (2000) (PubMed).
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- 抗原
- Alpha, beta-Tubulin Dimer
- 背景
- The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain. The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.,TUBA, TUBB
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