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Cytokeratins 抗体 (PE)

This anti- antibody is a 小鼠单克隆 antibody detecting in ICFC. Suitable for 哺乳动物. This Primary Antibody has been cited in 5+ publications.

Separation of HeLa cells from human lymphocytes stained using anti-human Cytokeratin (pan-reactive) (C-11) PE antibody (concentration in sample 5 μg/mL 100 μL of cell suspension, red-filled) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with HeLa cells. (Cytokeratins 抗体 (PE))

Intracellular Flow Cytometry analysis Intracellular flow cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using anti-cytokeratin antibody (C-11) PE. (Cytokeratins 抗体 (PE))

Detection of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody (Cytokeratins 抗体 (PE))

3 images

(5 references)

产品编号 ABIN192048

发货至: 中国

Quick Overview for Cytokeratins 抗体 (PE) (ABIN192048)

抗原

Cytokeratins

适用

哺乳动物

宿主

小鼠

克隆类型

单克隆

标记

PE

应用范围

Intracellular Flow Cytometry (ICFC)

克隆位点

C-11

原理

Anti-Cytokeratins PE

特异性

The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.

交叉反应（详细）

Mammalian

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

亚型

IgG1
anti-Cytokeratins antibody
适用: 哺乳动物 ICFC, ICC, IHC (p), IP, WB 宿主: 小鼠 Monoclonal C-11 unconjugated

anti-Cytokeratins antibody (FITC)
适用: 哺乳动物 ICFC 宿主: 小鼠 Monoclonal C-11 FITC

anti-Cytokeratins antibody (APC)
适用: 哺乳动物 ICFC 宿主: 小鼠 Monoclonal C-11 APC

anti-Cytokeratins antibody (Biotin)
适用: 哺乳动物 ICC, IHC (p), IP, WB, FACS 宿主: 小鼠 Monoclonal C-11 Biotin

应用备注

Flow cytometry: Recommended dilution: 1 μg/mL. Intracellular staining.

限制

仅限研究用
浓度

0.1 mg/mL

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Petry, Kippenberger, Meissner, Kleemann, Kaufmann, Rieger, Wellenbrock, Reichenbach, Zöller, Valesky: "Directing adipose-derived stem cells into keratinocyte-like cells: impact of medium composition and culture condition." in: Journal of the European Academy of Dermatology and Venereology : JEADV, Vol. 32, Issue 11, pp. 2010-2019, (2019) (PubMed).

Broekema, Harmsen, Koerts, Petersen, van Luyn, Navis, Popa: "Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats." in: Kidney international, Vol. 68, Issue 6, pp. 2572-81, (2005) (PubMed).

Hamakawa, Sumida, Tanioka, Sogawa, Yamada: "Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis." in: Research communications in molecular pathology and pharmacology, Vol. 101, Issue 2, pp. 115-26, (1999) (PubMed).

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).
抗原

Cytokeratins

背景

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,cytokeratin, CYK, CK, KRT

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