OSBPL5 抗体 (N-Term)
Quick Overview for OSBPL5 抗体 (N-Term) (ABIN190918)
抗原
See all OSBPL5 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- N-Term
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原理
- ORP5 (OBPH1)
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序列
- KEEAFLRRRF SLC
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特异性
- This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1, NP_001137535.1) encode the same isoform.
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交叉反应
- 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence KEEAFLRRRFSLC, from the N Terminus of the protein sequence according to NP_065947.1, NP_663613.1, NP_001137535.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Kidney and Liver. Recommended concentration: 5 μg/mL.
Western Blot: Approx. 100 kDa band observed in lysates of cell line Jurkat and in Rat Spleen lysates (calculated MW of 98.4 kDa according to Human NP_065947.1 and 101.7 kDa according to Rat NP_001015024.2). Recommended concentration: 0.03=0.3 μg/mL. Primary
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- OSBPL5 (Oxysterol Binding Protein-Like 5 (OSBPL5))
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别名
- OSBPL5
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背景
- OSBPL5, ORP5, OBPH1, FLJ31948, KIAA1534, DKFZp586H1719, oxysterol binding protein-like 5, OSBP-related protein 5, oxysterol-binding protein homologue 1, oxysterol-binding protein-related protein 5, FLJ42929, oxysterol-binding protein-like protein 5
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基因ID
- 114879, 79196
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NCBI登录号
- NP_065947, NP_663613, NP_001137535
抗原
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