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OSBPL5 抗体 (N-Term)

This 山羊 多克隆 antibody specifically detects OSBPL5 in WB, ELISA, IF, FACS 和 IHC. It exhibits reactivity toward 人 和 大鼠.
产品编号 ABIN190918
发货至: 中国
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Quick Overview for OSBPL5 抗体 (N-Term) (ABIN190918)

抗原

See all OSBPL5 抗体
OSBPL5 (Oxysterol Binding Protein-Like 5 (OSBPL5))

适用

人, 大鼠

宿主

  • 3
  • 2
山羊

克隆类型

  • 5
多克隆

标记

  • 5
This OSBPL5 antibody is un-conjugated

应用范围

  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)

质量等级

Verified
  • 抗原表位

    • 1
    • 1
    • 1
    N-Term

    原理

    ORP5 (OBPH1)

    序列

    KEEAFLRRRF SLC

    特异性

    This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1, NP_001137535.1) encode the same isoform.

    交叉反应

    犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence KEEAFLRRRFSLC, from the N Terminus of the protein sequence according to NP_065947.1, NP_663613.1, NP_001137535.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Kidney and Liver. Recommended concentration: 5 μg/mL.

    Western Blot: Approx. 100 kDa band observed in lysates of cell line Jurkat and in Rat Spleen lysates (calculated MW of 98.4 kDa according to Human NP_065947.1 and 101.7 kDa according to Rat NP_001015024.2). Recommended concentration: 0.03=0.3 μg/mL. Primary

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    OSBPL5 (Oxysterol Binding Protein-Like 5 (OSBPL5))

    别名

    OSBPL5

    背景

    OSBPL5, ORP5, OBPH1, FLJ31948, KIAA1534, DKFZp586H1719, oxysterol binding protein-like 5, OSBP-related protein 5, oxysterol-binding protein homologue 1, oxysterol-binding protein-related protein 5, FLJ42929, oxysterol-binding protein-like protein 5

    基因ID

    114879, 79196

    NCBI登录号

    NP_065947, NP_663613, NP_001137535
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