DGAT1 抗体 (Internal Region)
Quick Overview for DGAT1 抗体 (Internal Region) (ABIN190886)
抗原
See all DGAT1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- DGAT1
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序列
- QNSMKPFKDM DYS
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交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-QNSMKPFKDMDYS, from the internal region of the protein sequence according to NP_036211.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 55 kDa band observed in Mouse Liver lysates (calculated MW of 56.8 kDa according to NP_034176.1). Recommended concentration: 0.01-0.1.0 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:64000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommende
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))
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别名
- DGAT1
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背景
- DGAT1, diacylglycerol O-acyltransferase homolog 1 (mouse) , ARGP1, DGAT , ACAT related gene product 1, acyl coenzyme A:cholesterol acyltransferase related gene 1, diacylglycerol O-acyltransferase 1, diglyceride acyltransferase
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基因ID
- 8694, 13350, 84497
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NCBI登录号
- NP_036211
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途径
- Hormone Transport
抗原
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