ABHD5 抗体 (Internal Region)
Quick Overview for ABHD5 抗体 (Internal Region) (ABIN190850)
抗原
See all ABHD5 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- CGI58 / ABHD5
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序列
- FPERPDLADQ DR
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特异性
- This antibody is not expected to cross-react with ABHD4.
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交叉反应
- 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-FPERPDLADQDR, from the internal region of the protein sequence according to NP_057090.2.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Pancreas and Kidney. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 40 kDa band observed in lysates of cell lines NIH3T3/3TL (calculated MW of 39.1 kDa according to NP_057090.2). Additional faint bands of 26 kDa and 18 kDa were also observed, and were successfully blocked by incubation with the immunizin
Peptide ELISA: antibody detection limit dilution 1:16000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm/ vesicles of U2OS cells. Recommended concentration: 10μg/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Coordination of lipid droplet-associated proteins during the transition period of Holstein dairy cows." in: Journal of dairy science, Vol. 94, Issue 4, pp. 1839-48, (2011) (PubMed).
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: "Coordination of lipid droplet-associated proteins during the transition period of Holstein dairy cows." in: Journal of dairy science, Vol. 94, Issue 4, pp. 1839-48, (2011) (PubMed).
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- ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))
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别名
- ABHD5
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背景
- ABHD5, CGI58 protein, aCGI58, bhydrolase domain containing 5, CDS, IECN2, MGC8731, NCIE2
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基因ID
- 51099, 67469, 316122
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NCBI登录号
- NP_057090
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途径
- Lipid Metabolism
抗原
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