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ABHD5 抗体 (Internal Region)

There is 1 publication for this product available. The 山羊 多克隆 anti-ABHD5 antibody is suitable to detect ABHD5 in samples from 人 和 小鼠. It has been validated for WB, ELISA, IHC 和 IF.
产品编号 ABIN190850
发货至: 中国
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Quick Overview for ABHD5 抗体 (Internal Region) (ABIN190850)

抗原

See all ABHD5 抗体
ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))

适用

  • 46
  • 9
  • 9
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 1
人, 小鼠

宿主

  • 38
  • 9
山羊

克隆类型

  • 26
  • 21
多克隆

标记

  • 25
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
This ABHD5 antibody is un-conjugated

应用范围

  • 34
  • 19
  • 13
  • 6
  • 6
  • 5
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    原理

    CGI58 / ABHD5

    序列

    FPERPDLADQ DR

    特异性

    This antibody is not expected to cross-react with ABHD4.

    交叉反应

    犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-FPERPDLADQDR, from the internal region of the protein sequence according to NP_057090.2.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Pancreas and Kidney. Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 40 kDa band observed in lysates of cell lines NIH3T3/3TL (calculated MW of 39.1 kDa according to NP_057090.2). Additional faint bands of 26 kDa and 18 kDa were also observed, and were successfully blocked by incubation with the immunizin

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm/ vesicles of U2OS cells. Recommended concentration: 10μg/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Koltes, Spurlock: "Coordination of lipid droplet-associated proteins during the transition period of Holstein dairy cows." in: Journal of dairy science, Vol. 94, Issue 4, pp. 1839-48, (2011) (PubMed).

  • 抗原

    ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))

    别名

    ABHD5

    背景

    ABHD5, CGI58 protein, aCGI58, bhydrolase domain containing 5, CDS, IECN2, MGC8731, NCIE2

    基因ID

    51099, 67469, 316122

    NCBI登录号

    NP_057090

    途径

    Lipid Metabolism
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