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PAX6 抗体 (Internal Region)

This anti-PAX6 antibody is a 山羊 多克隆 antibody detecting PAX6 in WB, ELISA 和 FACS. Suitable for 人 和 小鼠.
产品编号 ABIN190794
发货至: 中国

Quick Overview for PAX6 抗体 (Internal Region) (ABIN190794)

抗原

See all PAX6 抗体
PAX6 (Paired Box 6 (PAX6))

适用

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人, 小鼠

宿主

  • 94
  • 44
山羊

克隆类型

  • 79
  • 58
多克隆

标记

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  • 1
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This PAX6 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    PAX6 (internal)

    序列

    REEKLRNQRR QASN

    特异性

    This antibody is expected to recognize all reported isoforms: NP_000271.1, NP_001595.2, NP_001297088.1, NP_001355839.1, NP_001355840.1, NP_001355841.1, NP_001355844.1, NP_001355847.1, NP_001355849.1, NP_001355850.1, NP_001355851.1, NP_001355857.1, NP_0013

    交叉反应

    Cow, 犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-REEKLRNQRRQASN, from the internal region of the protein sequence according to NP_000271.1, NP_001595.2.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 26 kDa band observed in lysates of cell lines U251 and RAW264.7 and in nuclear lysates of cell lines K562 and HeLa, all successfully blocked by incubation with the immunising peptide (calculated MW of 24.8 kDa according to Human NP_001

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    PAX6 (Paired Box 6 (PAX6))

    别名

    PAX6

    背景

    PAX6, paired box gene 6 (aniridia, keratitis), AN, AN2, D11S812E, MGC17209, MGDA, WAGR, paired box gene 6, paired box homeotic gene 6 (aniridia, keratitis), paired box homeotic gene-6

    基因ID

    5080, 18508, 25509

    NCBI登录号

    NP_000271, NP_001595

    途径

    Peptide Hormone Metabolism, Carbohydrate Homeostasis
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