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ILF2 抗体 (Internal Region)

This anti-ILF2 antibody is a 山羊 多克隆 antibody detecting ILF2 in WB 和 ELISA. Suitable for 人. This Primary Antibody has been cited in 2+ publications.
产品编号 ABIN190786
发货至: 中国

Quick Overview for ILF2 抗体 (Internal Region) (ABIN190786)

抗原

See all ILF2 抗体
ILF2 (Interleukin Enhancer Binding Factor 2, 45kDa (ILF2))

适用

  • 54
  • 26
  • 25
  • 8
  • 6
  • 5
  • 5
  • 5
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2

宿主

  • 47
  • 6
  • 1
山羊

克隆类型

  • 42
  • 12
多克隆

标记

  • 27
  • 6
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
This ILF2 antibody is un-conjugated

应用范围

  • 36
  • 21
  • 18
  • 6
  • 5
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA

质量等级

Verified
  • 抗原表位

    • 9
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    原理

    ILF2 / NF45

    序列

    EKAYEKPPEK KEG

    特异性

    This antibody is expected to recognize both reported isoforms (NP_004506.2, NP_001254738.1).

    交叉反应

    犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-EKAYEKPPEKKEG, from the internal region (near the C Terminus) of the protein sequence according to NP_004506.2.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 43 kDa band observed in Human Tonsil and Thymus lysates (calculated MW of 43.1 kDa according to NP_004506.2). Recommended concentration: 0.03-0.1 μg/mL. This antibody has been successfully used in WB in the following papers: Ye J et al

    Peptide ELISA: antibody detection limit dilution 1:4000.

    说明

    Immunoprecipitation: This antibody was confirmed by a customer in a special IP procedure called PAR-CLIP (see for method PMID: 20644507). This data is from a previous batch, not on sale.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Shamanna, Hoque, Peery, Mathews: "Induction of p53, p21 and apoptosis by silencing the NF90/NF45 complex in human papilloma virus-transformed cervical carcinoma cells." in: Oncogene, Vol. 32, Issue 43, pp. 5176-85, (2013) (PubMed).

    Shamanna, Hoque, Lewis-Antes, Azzam, Lagunoff, Peery, Mathews: "The NF90/NF45 complex participates in DNA break repair via nonhomologous end joining." in: Molecular and cellular biology, Vol. 31, Issue 23, pp. 4832-43, (2011) (PubMed).

  • 抗原

    ILF2 (Interleukin Enhancer Binding Factor 2, 45kDa (ILF2))

    别名

    ILF2

    背景

    ILF2, NF45, interleukin enhancer binding factor 2, 45 kDa , MGC8391, PRO3063 , interleukin enhancer binding factor 2, nuclear factor of activated T-cells, 45- kDa

    基因ID

    3608, 67781, 310612

    NCBI登录号

    NP_004506, NP_001254738
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