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Glucose-6-Phosphate Dehydrogenase 抗体 (AA 308-320)

Cited in 1 publication. This 山羊 多克隆 anti-Glucose-6-Phosphate Dehydrogenase antibody specifically detects Glucose-6-Phosphate Dehydrogenase in WB, IHC 和 ELISA. The antibody is reactive with 人 samples.
产品编号 ABIN190766
发货至: 中国
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Quick Overview for Glucose-6-Phosphate Dehydrogenase 抗体 (AA 308-320) (ABIN190766)

抗原

See all Glucose-6-Phosphate Dehydrogenase (G6PD) 抗体
Glucose-6-Phosphate Dehydrogenase (G6PD)

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This Glucose-6-Phosphate Dehydrogenase antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA

质量等级

Verified
  • 抗原表位

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    AA 308-320

    原理

    G6PD (aa 308 - 320)

    序列

    STNSDDVRDE KVK

    特异性

    This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).

    交叉反应

    犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-STNSDDVRDEKVK, from the internal region of the protein sequence according to NP_000393.4 , NP_001035810.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Testis and Spleen. Recommended concentration: 2.5 μg/mL.

    Western Blot: Approx 55-60 kDa double band observed in Human Testis, Thyroid and Tonsil lysates (calculated MW of 62.5 kDa according to NP_000393.4 and 59.3 kDa accordiong to NP_001035810.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Hwang, Mruk, Rahighi, Raub, Chen, Dorn, Horikoshi, Wakatsuki, Chen, Mochly-Rosen: "Correcting glucose-6-phosphate dehydrogenase deficiency with a small-molecule activator." in: Nature communications, Vol. 9, Issue 1, pp. 4045, (2019) (PubMed).

  • 抗原

    Glucose-6-Phosphate Dehydrogenase (G6PD)

    别名

    G6PD

    背景

    G6PD, glucose-6-phosphate dehydrogenase, G6PD1, glucose-6-phosphate 1-dehydrogenase, glucose-6-phosphate dehydrogenase, G6PD

    基因ID

    2539, 14381, 24377

    NCBI登录号

    NP_000393, NP_001035810

    途径

    Regulation of Systemic Arterial Blood Pressure by Hormones
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