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FH 抗体 (Internal Region)

This 山羊 多克隆 anti-FH antibody specifically detects FH in WB, ELISA, IHC 和 EIA. The antibody is reactive with 人 samples.
产品编号 ABIN190758
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for FH 抗体 (Internal Region) (ABIN190758)

抗原

See all FH 抗体
FH (Fumarate Hydratase (FH))

适用

  • 105
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宿主

  • 83
  • 28
  • 1
山羊

克隆类型

  • 74
  • 38
多克隆

标记

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This FH antibody is un-conjugated

应用范围

  • 77
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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

质量等级

Verified
  • 抗原表位

    • 15
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    Internal Region

    原理

    Fumarase / FH

    序列

    HPNDHVNKSQ SSND

    预测反应

    Human, Mouse, Rat, Dog

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-HPNDHVNKSQSSND, from the internal region of the protein sequence according to NP_000134.2.

    亚型

    IgG
  • 应用备注

    DS WB Results: Approx 48 kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6 kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03 μg/mL.

    DS IHC Results: Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 3.75 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Fumarase captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    FH (Fumarate Hydratase (FH))

    别名

    Fumarate Hydratase

    背景

    Fumarate hydratase, HLRCC, LRCC, MCL, MCUL1, fumarase, leiomyomatosis and renal cell cancer, multiple hereditary cutaneous leiomyomata

    基因ID

    2271

    NCBI登录号

    NP_000134
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