PsbP 抗体
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北京 101111
Quick Overview for PsbP 抗体 (ABIN190744)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-protein)
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交叉反应 （详细）
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Arabidopsis thaliana, Chlamydomonas reinhardti, Hordeum vulgare, Pinus banksiana, Spinacia oleracea, Triticum aestivum
Not reactive in: Synechococcus sp. PCC 7942 -
预测反应
- Musa acuminata,Oryza sativa,Pinus monticola,Pisum sativum,Populus balsamifera,Solanum lycopersicum
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纯化方法
- Protein G purified
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免疫原
- native
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anti-PsbP antibody
适用: Arabidopsis thaliana, Barley, Nicotiana tabacum, Pea, Physcomytrium Patens, Picea abies WB 宿主: 兔 Polyclonal unconjugated
anti-PsbP antibody适用: Arabidopsis thaliana, Sorghum, Zea mays WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1 : 2000-1 : 5000 (WB)
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说明
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Load per well on cell extract of Pinus banksiana (Jack Pine) was 7 µg.This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 200 μL of sterile water
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缓冲液
- Total IgG. Protein G purified in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- PsbP
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背景
- PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33- kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.
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分子量
- 28 | 23 kDa
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UniProt
- P12302
抗原
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