PsbP 抗体
(2 references)-
- 抗原
- PsbP
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适用
- Arabidopsis thaliana, Barley, Spinach
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- Western Blotting (WB)
- 交叉反应 （详细）
- No cross-reactivity with: Synechococcus
- 预测反应
- dicots including: Pisum sativum, Solanum lycopersicum, monocots including: Oryza sativa, trees including: Populus balsamifera, Pinus monticola
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 28 / 23 kDa
- 纯化方法
- affinity purified
- 免疫原
- native, purified 23 kDa protein from Spinacia oleracea
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anti-PsbP antibody
适用: Arabidopsis thaliana, Barley, Moss, Nicotiana tabacum, Pisum sativum WB 宿主: 兔 Polyclonal unconjugated
anti-PsbP antibody适用: Arabidopsis thaliana, Sorghum, Zea mays WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Recommended Dilution 1 : 2000 - 1 : 5000 with standard ECL (WB).
- 说明
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Load per well on cell extract of Pinus banksiana (Jack Pine) was 7 µg.This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS pH 7.4
- 注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
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- 抗原
- PsbP
- 背景
- PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.
- 分子量
- expected: 28 kDa, apparent: 23 kDa
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