Nitrogenase 抗体
This anti-Nitrogenase antibody (ABIN190742) is a Chicken Polyclonal antibody detecting Nitrogenase in WB. Suitable for Rhodopseudomonas palustris.
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(2 references)Quick Overview for Nitrogenase 抗体 (ABIN190742)
抗原
Nitrogenase (Nifh)
适用
Rhodopseudomonas palustris
宿主
小鸡
克隆类型
多克隆
应用范围
Western Blotting (WB)
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交叉反应 （详细）
- No cross-reactivity with: Synechococcus sp. PCC 7942 and Synechocystis
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预测反应
- Desulfotomaculum reducens (strain MI-1),Clostridium cellobioparum, Frankia sp. (strain EAN1pec), Magnetococcus sp., Methanobacterium thermoautotrophicum, Methanococcus maripaludis, Methylobacterium sp , Rhodopseudomonas palustris TIE-1 strain, alpha,gamma,beta proteobacteria, enterobacteria, low GC gram+, high GC gram +, euryachaeotes, Azotobacter vinelandii (Gram-), Klebsiella pneumonia, cyanobacteria, Enterobacter genera, able to fix atmoshperic nitrogen
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产品特性
- Expected / apparent Molecular Weight of the Antigene: 27 / 32.5 kDa
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纯化方法
- affinity purified
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免疫原
- KLH-conjugated synthetic peptide derived from known bacterial NifH subunits of bacterial nitrogenase enzymes of the FeMoCo type including Synechoccocus sp. Q2JP78 , Trichodesmium theibautii, Anabaena sp. P33178 and Nostoc sp. Q51296
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应用备注
- Recommended Dilution 1 : 2 000 (WB).
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说明
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This antibody is not suitable for immunolocalization studies on bacterial cultures.An enzyme involved in chlorophyll synthesis, present in all cyanobacteria (fixing and non-nitrogen fixing) is a member of the NifH family/superfamily. Agrisera anti-NifH antibody will not show a strong reactivity to this target.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.36 µg/µL
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缓冲液
- PBS pH 8.0+ 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Make aliquots to avoid repreated freeze-thaw cycles and working with a stock. -
储存条件
- 4 °C
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: "Fatty alcohols can complement functions of heterocyst specific glycolipids in Anabaena sp. PCC 7120." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 178-83, (2014) (PubMed).
: "Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations." in: Environmental microbiology, Vol. 12, Issue 7, pp. 1899-912, (2010) (PubMed).
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: "Fatty alcohols can complement functions of heterocyst specific glycolipids in Anabaena sp. PCC 7120." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 178-83, (2014) (PubMed).
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- Nitrogenase (Nifh)
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背景
- Nitrogenase is involved in biological fixation of atmospheric nitrogen to ammonia. Alternative protein names: nitrogenase component II, nitrogenase Fe protein, nitrogenase reductase, FeMoCo-nitrogenase.
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分子量
- expected: 27 kDa, apparent: 32.5 kDa
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UniProt
- P26254, Q2JP78, P33178, Q51296
抗原
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