Nitrogenase 抗体
There are 2+ publications for this product available. The 小鸡 多克隆 anti-Nitrogenase antibody is suitable to detect Nitrogenase in samples from Anabaena variabilis, Clostridium butyricum, Codakia orbicularis, Cylindrospermopsis raciborskii, Dolichospermum, Nostoc, Rhodopseudomonas palustris, Trichodesmium, Trifolium repens 和 Vibrio sp.. It has been validated for IF, IHC 和 WB.
1 image
(2 references)
产品编号 ABIN190742
发货至:
中国
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Our Local Distributor
中国
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北京 101111
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Quick Overview for Nitrogenase 抗体 (ABIN190742)
抗原
Nitrogenase (Nifh)
适用
Anabaena variabilis, Clostridium butyricum, Codakia orbicularis, Cylindrospermopsis raciborskii, Dolichospermum, Nostoc, Rhodopseudomonas palustris, Trichodesmium, Trifolium repens, Vibrio sp.
宿主
小鸡
克隆类型
多克隆
应用范围
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
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原理
- Anti-NifH | Nitrogenase iron protein
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交叉反应 （详细）
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Anabaena PCC7120, Clostridium butyricum, Codakia orbicularis, Cylindrospermopsis raciborskii CS-505, Dolichospermum sp., Nostoc sp, Rhodopseudomonas palustris, Trichodesmium sp., nodules of Trifolium repens L., Vibrio natriegens ATCC 14048
Not reactive in: Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803 as NifH protein is not present in those cyanobacterial species, Frankia sp. -
预测反应
- Azotobacter vinelandii (Gram-),Bradyrhizobium japonicum,Cyanobacteria,Cyanothece ATCC51142,Desulfotomaculum reducens (strain MI-1),Clostridium cellobioparum,Enterobacter,genera,euryachaeotes,Klebsiella pneumonia,Magnetococcus sp.,Methanobacterium thermoautotrophicum,Methanococcus maripaludis,Methylobacterium sp.,Mesoorhizobium loti,Rhodopseudomonas palustris TIE-1 strain,alpha,gamma,beta proteobacteria,enterobacteria,low GC gram+,high GC gram +,able to fix atmoshperic nitrogen,Rhizobium meliloti
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纯化方法
- Immunogen affinity purified
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免疫原
- KLH-conjugated synthetic peptide derived from known bacterial NifH subunits of bacterial nitrogenase enzymes of the FeMoCo type including Synechoccocus sp. Q2JP78
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应用备注
- 1 : 500 (IHC), 6 μg/mL (IF), 1 : 2000 (WB)
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说明
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This antibody is not suitable for immunolocalization studies on bacterial cultures.An enzyme involved in chlorophyll synthesis, present in all cyanobacteria (fixing and non-nitrogen fixing) is a member of the NifH family/superfamily. The anti-NifH antibody will not show a strong reactivity to this target.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.36 µg/µL
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缓冲液
- Immunogen affinity purified IgY in PBS pH 8 and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- 4 °C
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储存方法
- Store at 4°C, make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Fatty alcohols can complement functions of heterocyst specific glycolipids in Anabaena sp. PCC 7120." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 178-83, (2014) (PubMed).
: "Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations." in: Environmental microbiology, Vol. 12, Issue 7, pp. 1899-912, (2010) (PubMed).
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: "Fatty alcohols can complement functions of heterocyst specific glycolipids in Anabaena sp. PCC 7120." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 178-83, (2014) (PubMed).
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