Ftsz 抗体
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北京 101111
Quick Overview for Ftsz 抗体 (ABIN190734)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-FtsZ | Procaryotic cell division GTPase (cyanobacterial)
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特异性
- Antibodies will also recognize FtsZ protein from E.coli strain BL21.
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交叉反应 （详细）
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Cylindrospermopsis raciborskii CS-505, Listeria monocytogenes (weak reaction), Synechococcus elongatus
Not reactive in: higher plants -
预测反应
- Phaeodactylum tricornutum,Prochlorococcus sp.
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纯化方法
- Serum
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免疫原
- Whole cyanobacterial (Anabaena PCC 7120) FtsZ protein
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anti-Procaryotic Cell Division Gtpase (Ftsz) antibody
Ftsz 适用: Candidatus Moranella endobia, E. coli, Shigella flexneri IF, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1 : 200 (IL) Immunogold-TEM, 1 : 500 (IF), 1 : 2000-1 : 5000 (WB)
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说明
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For western blot detection image and immunolocalization results - please refer to the article below.Immunofluorescence has been done by labelling Synechococcus elongatus cells at 30°C for 2 hours with FtsZ antibodies diluted to 1: 500 in blocking buffer. Detection images can be found in Kabeya et al (2010).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- Ftsz (Procaryotic Cell Division Gtpase (Ftsz))
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背景
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Background: FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria as well as in chloroplasts. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules.
Additional information: To detect E.coli FtsZ protein we recommend a following product: AS10 715 | anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibodyTo detect FtsZ protein in higher plants following antibodies are recommended: AS09 413 | Anti-FtsZ1 and 2 | Plant cell division protein FtsZ1 and FtsZ2, rabbit antibodiesAS13 2651 | Anti-FtsZ2 | Plant cell division protein ftsZ2, rabbit antibodies
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分子量
- 44.5 | 50 kDa
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UniProt
- Q3MC27
抗原
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